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Protective Effect of α-ketobutyrate on Survival of Hippocampal Neurons Challenged with Hydrogen Peroxide Chemistry Mimicking Brain Ischemia

[ Vol. 10 , Issue. 2 ]

Author(s):

Dagmara Jacewicz, Kamila Siedlecka-Kroplewska, Joanna Pranczk, Urszula Popowska, Alicja Kuban-Jankowska, Narcyz Knap, Dariusz Wyrzykowski, Krzysztof Zamojc, Michal Wozniak and Lech Chmurzyrski   Pages 87 - 91 ( 5 )

Abstract:


A protective effect of α -ketobutyrate on hippocampal neuronal cells treated with H2O2 was tested. α - Ketobutyrate concentrations were as follows: 0.5 mM, 1 mM and 5 mM. Tests were performed at the temperature of 37 ºC. Moreover, the protective effect of α-ketobutyrate on the cytotoxicity of 100 and 40 nmol/4·103 cells treated by hydrogen peroxide was observed. Furthermore, the role of α-ketobutyrate as an energy substrate was also proven using the cis- [Cr(C2O4)(pm)(OH2)2]+ complex as a biosensor for the carbon dioxide uptake process. All the experiments were carried out under in-vitro conditions.

Keywords:

Neuronal cells, molecular biosensor of CO2, chromium(III) complex.

Affiliation:

Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdańsk, Poland.



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