Osmar dos Reis Antunes Junior, Emilli Antônio, Rubiana M. Mainardes and Najeh M. Khalil Pages 121 - 128 ( 8 )
The objective of this work was to develop and validate a rapid and effective chromatographic procedure for the quantitative analysis of diphenyl diselenide in poly(lactide) (PLA) nanoparticles via reversed-phase high performance liquid chromatography (RP-HPLC), using a photodiode array detector (PDA). Chromatographic runs were performed on a RP C18 column (125 mm, 4.0 mm, 5 µm) with a mixture of methanol and acidified water 0.5% (90:10, v/v) as the mobile phase and a flow rate of 1.0 mL/min in the isocratic mode. Diphenyl diselenide was detected at a wavelength 240 nm. This method was shown to be specific and linear in the range 10-100 µg/mL (r =0.999). The limits of detection and quantitation were 0.219 and 0.666 µg/mL, respectively. The method was robust for changes in the mobile phase and flow rate. The precision (intra- and inter-day) was demonstrated because the maximum relative standard deviation was 3.52%. The mean recovery for diphenyl diselenide was 99.54% (relative standard deviation = 0.44, n = 9). The method was successfully used to determine the encapsulation efficiency of diphenyl diselenide in PLA nanoparticles.
Diphenyl diselenide, encapsulation efficiency, HPLC, polymeric nanoparticles, validation.
Department of Pharmacy, Laboratory of Pharmaceutical Nanotechnology, Universidade Estadual do Centro-Oeste/UNICENTRO, Rua Simeão Camargo Varela de Sá 03, 85040- 080 Guarapuava, PR – Brazil.