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A Liquid–Liquid Extraction–Assisted HPLC Procedure for Assaying of Citalopram in Depressed Women Saliva

[ Vol. 13 , Issue. 4 ]


Ewelina Dziurkowska and Marek Wesolowski*   Pages 352 - 360 ( 9 )


Background: A search of the relevant literature shows that there is still a paucity of information on the theme of saliva as a diagnostic material for the quantitation of antidepressants. The objective of this study, therefore, was to develop and validate a sensitive, reliable and rapid HPLC procedure for the quantitation of citalopram in the saliva of women with major depressive disorders who were undergoing citalopram therapy.

Method: A single–step liquid–liquid extraction (LLE) with dichloromethane was used for the isolation of citalopram from saliva. For its quantitation a HPLC system was used.

Result: The procedure developed was validated for selectivity, linearity, limits of detection and quantitation, precision, accuracy and stability. Single-step LLE with dichloromethane was found to be more profitable than other techniques. The extractant ensures isolation of the antidepressant and internal standard with satisfactory inter–day and intra–day precision and accuracy.

Conclusion: The procedure enabled low quantities of citalopram to be quantified in saliva using HPLC with a simple UV detector. Single-step LLE was found to be a convenient, workable and cost– effective tool for purifying saliva. Saliva used as a diagnostic material has the benefit of reducing the anxiety associated with blood sampling, making the proposed procedure stress–free and non–invasive for depressed patients. Moreover, it can be helpful in monitoring the intake of medicine by subjects with apathy or depression.


HPLC quantitation, citalopram, saliva, drug monitoring under therapy, carbamazepine.


Department of Analytical Chemistry, Medical University of Gdansk, Gen. J. Hallera 107, 80-416 Gdansk, Department of Analytical Chemistry, Medical University of Gdansk, Gen. J. Hallera 107, 80-416 Gdansk

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