Article Details


Solid Phase Micro Membrane Tip Extraction and Capillary Electrophoresis of Metformin in Urine Samples

[ Vol. 13 , Issue. 4 ]

Author(s):

Mohammed Fahad Alajmi, Afzal Hussain, Syed Noeman Taqui and Imran Ali   Pages 403 - 409 ( 7 )

Abstract:


Background: Metformin is very important anti-hyperglycemic drug for non-insulin reliant diabetes mellitus. It excretes through urine. Therefore, its analysis is an important issue; especially with versatile capillary electrophoresis method.

Introduction: Inexpensive solid phase micro membrane tip extraction (SPMMTE) and capillary electrophoretic (CE) methods were presented for monitoring of metformin in urine sample.

Method: Iron nano composite adsorbent was prepared by green chemistry for SPMMTE. CE experimental conditions were fused silica capillary (60 cm x 50 µm I.D.), phosphate buffer (50 mM, 3.0 pH) as back ground electrolyte (BGE), 20 kV applied voltage, 254 nm detection, 20 seconds injection time and 27±1 ºC working temperature.

Results: The values of electrophoretic mobility, electroosmotic mobility, migration time, number of theoretical plates and velocity were 2.71 x 10-2 cm2V-1s-1, 3.50 x 10-4 cm2V-1s-1, 10.05 minutes, 14,400 and 5.42 cm min-1, correspondingly. The % recoveries of metformin in the laboratory prepared solution in water and urine samples were 90 and 80%.

Conclusion: It was observed that the developed SPMMTE and CE methods may be used to analyse metformin in any urine sample.

Keywords:

Fe nano composite adsorbent, solid phase membrane tip extraction, capillary electrophoresis, metformin, urine sample, analysis.

Affiliation:

Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Department of Chemistry, Jamia Millia Islamia (Central University), New Delhi-110025, Department of Chemistry, Jamia Millia Islamia (Central University), New Delhi-110025

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