Article Details


Development and Validation of a Simple High-Performance Liquid Chromatography Method for Estimation of Felodipine in Rabbit Plasma: Application to Pharmacokinetic Study

[ Vol. 13 , Issue. 4 ]

Author(s):

Murali Monohar Pandey, Shrikant Y. Charde, A. Jaipal, Prashant P. Raut, Puneet Goel and Love Kumar   Pages 321 - 327 ( 7 )

Abstract:


Background: A new, simple, specific and sensitive reverse-phase liquid chromatographic method was developed and validated for the estimation of felodipine (FDP) in rabbit plasma using fluorescence detector.

Materials and Methods: Plasma standard were prepared using a simple and one-step protein precipitation method and 50 μ l of the standard solutions were injected into the column. The method was found to be linear in the concentration range of 10–1000 ng/ml. The developed bioanalytical method was validated as per standard guidelines. Validation study results demonstrated accuracy, precision, sensitivity and selectivity of the proposed method.

Results: The developed method was successfully employed in the determination of FDP in plasma samples obtained from the pharmacokinetic study of the immediate release tablets in rabbits.

Keywords:

Felodipine, fluorescence detector, high-performance liquid chromatography, rabbit plasma, pharmacokinetic study, oral tablet.

Affiliation:

Industrial Research Laboratory, Department of Pharmacy, Birla Institute of Technology & Science, Pilani, Rajasthan- 333031, A-005, Department of Pharmacy, Birla Institute of Technology & Science, Pilani, Hyderabad Campus, Hyderabad: 500078, Telangana, Industrial Research Laboratory, Department of Pharmacy, Birla Institute of Technology & Science, Pilani, Rajasthan- 333031, Industrial Research Laboratory, Department of Pharmacy, Birla Institute of Technology & Science, Pilani, Rajasthan- 333031, Industrial Research Laboratory, Department of Pharmacy, Birla Institute of Technology & Science, Pilani, Rajasthan- 333031, Industrial Research Laboratory, Department of Pharmacy, Birla Institute of Technology & Science, Pilani, Rajasthan- 333031

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