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Methods to Evaluate the Activity of Nitric Oxide Synthase

[ Vol. 13 , Issue. 5 ]

Author(s):

Marialuigia Fantacuzzi, Alessandra Ammazzalorso, Barbara De Filippis, Letizia Giampietro, Cristina Maccallini and Rosa Amoroso   Pages 411 - 416 ( 6 )

Abstract:


Objective: The most used techniques to evaluate the inhibitor activity of different compounds on Nitric Oxide Synthases are presented.

Method: Griess assay, oxy-hemoglobin capture assay, Radio citrulline assay, and RP-HPLC assay.

Result: Griess assay, and oxy-hemoglobin capture assay detect the nitric oxide produced by NOS; while radio citrulline assay, and RP-HPLC assay take into consideration the concentration of Lcitrulline as stoichiometric to nitric oxide.

Conclusion: Griess assay and oxy-hemoglobin capture assay are suitable for a first screening of NOS activity, while the others could be used for a more precise activity determination.

Keywords:

Nitric oxide synthase, nitric oxide, L-arginine, L-citrulline, griess assay, hemoglobin assay, citrulline assay, RPHPLC assay.

Affiliation:

Department of Pharmacy, “G. d’Annunzio” University, Chieti, Department of Pharmacy, “G. d’Annunzio” University, Chieti, Department of Pharmacy, “G. d’Annunzio” University, Chieti, Department of Pharmacy, “G. d’Annunzio” University, Chieti, Department of Pharmacy, “G. d’Annunzio” University, Chieti, Department of Pharmacy, “G. d’Annunzio” University, Chieti

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