Submit Manuscript  

Article Details


Development and Validation of a New HPLC Method For the Detection of 5- Fluorouracil in Mobile Phase and in Plasma

[ Vol. 14 , Issue. 1 ]

Author(s):

Muhammad Hanif*, Muhammad Faheem Akhtar*, Shabana Naeem, Muqeet Wahid, Muhammad Ahmad Shehzad, Malik Saadullah, Bushra Nasir and Samina Afzal   Pages 3 - 7 ( 5 )

Abstract:


Objective: To establish a particular, sensitive and accurate High Performance Liquid Chromatography (HPLC) technique for the specific and effective analysis of 5-Fluorouracil (5-FU) in mobile phase and in plasma.

Method: Analytical methods for 5-FU in mobile phase and plasma were developed. An assay consisted of isocratic elution of 5-FU in prepacked column discovery R-HS C-18, 5micrometer, Catalog # 568523-U, column dimensions : 25cm x 4.6mm using mobile phase composed of 5 mmol/L potassium dihydrogen phosphate (pH 6.0) and methanol at a ratio of 96:4 v/v respectively. 5-FU was detected at 254 nm with the flow rate of 1 ml/min in both mobile phase and plasma.

Results: Retention time was found to be 3.457 minutes in mobile phase and 2.543 minutes in plasma. System suitability test showed no robustness among the inter-day, intra-day analysis, mobile phase and column. Accuracy of the developed method proved reliability of the purposed procedure. %RSD analysis was found to be less than 2% and coefficient of correlation was found to be 1.

Conclusion: Valuable precision, recovery and accuracy were observed indicating method reliability. Method can also be applicable for bioequivalence study.

Keywords:

5-Fluorouracil, HPLC, mobile phase, plasma, linearity, method validation.

Affiliation:

Department of Pharmacy, Bahauddin Zakariya University, Multan, Department of Pharmacy, Bahauddin Zakariya University, Multan, Department of Pharmacy, Bahauddin Zakariya University, Multan, Department of Pharmacy, Bahauddin Zakariya University, Multan, Department of Statistics, Bahauddin Zakariya University, Multan, Faculty of Pharmacy Bahauddin Zakariya University, Multan, Faculty of Pharmacy Bahauddin Zakariya University, Multan, Faculty of Pharmacy Bahauddin Zakariya University, Multan

Graphical Abstract:



Read Full-Text article