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Analytical Method Development and Validation for Concurrent Determination of Bromohexine and Phenylpropanolamine by Reverse Phase-HPLC Method

[ Vol. 13 , Issue. 5 ]

Author(s):

Kishor Kumar Erukulla and S. R. Suseem   Pages 438 - 445 ( 8 )

Abstract:


Objective: A particular, easy and precise reverse phase liquid chromatographic technique was developed for the synchronous determination of Bromohexine and Phenylpropanolamine in pharmaceutical dose forms as there is no consolidated strategy accessible for the concurrent determination of Bromohexine and Phenylpropanolamine.

Methods: The BDS C18 (250mm x 4.6mm, 5µm) column was utilized in isocratic mode. The mobile phase contains phosphate buffer and acetonitrile (50:50 v/v). The buffer is set up by including 1ml of orthophosphoric acid in a 1000 ml of volumetric flask and 900 ml of milli-Q water added and degas to sonicate lastly make up the volume with water. The rate of flow was 1 ml/min. The effluents were observed at 250 nm.

Results:The observed retention time of Bromohexine was 2. 323 min and for Phenylpropanolamine 2. 719 min. The linearity values observed for bromohexine was of 8-48 µg/ml and for phenylpropanolamine, it was 25-150 µ g/ml. The observed recovery of Bromohexine was 98. 16 % and phenylpropanolamine was 101.29 %.

Conclusion: The proposed strategy was validated and the same is very useful for the synchronous determination of Bromohexine and Phenylpropanolamine.

Keywords:

Bromohexine, phenylpropanolamine, validation, method, buffer and development.

Affiliation:

Department of Chemistry, VIT University, Vellore, Tamilnadu, 632014, Department of Chemistry, VIT University, Vellore, Tamilnadu, 632014

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