Mohammad Jamal Amer Shammout and Nursabah Elif Basci* Pages 68 - 75 ( 8 )
Introduction: Chromatographic methods for determination of betamethasone or dexamethasone in their pharmaceutical preparations by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry were developed.
Method: Analytes were eluted from Acquity BEH C18 (50 mm×2.1 mm, 1.7 µm) column using stepwise gradient with mobile phase consisted of acetonitrile and 0.1% (v/v) acetic acid in water. Quantitation of dexamethasone and betamethasone was performed using 451>361 m/z on MRM mode.
Results: Methods were linear over the concentration range from 10 to 1500 ng mL -1 for both analyte. Limits of detection for BTM or DXM were 1 ng mL -1. The highest relative error and relative standard deviation values were not over 2%.
Conclusion: Presented methods were specific, rugged and robust, successfully applied to the analysis of their pharmaceutical preparations.
Dexamethasone, betamethasone, UPLC-MS/MS, tablet, ampoule, drops.
Analytical Chemistry Department, Faculty of Pharmacy, Hacettepe University, Ankara, Analytical Chemistry Department, Faculty of Pharmacy, Hacettepe University, Ankara