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Validated Ultra Performance Liquid Chromatography-Tandem Mass Spectrometric Method for Determination of Betamethasone or Dexamethasone in Pharmaceuticals

[ Vol. 14 , Issue. 1 ]

Author(s):

Mohammad Jamal Amer Shammout and Nursabah Elif Basci*   Pages 68 - 75 ( 8 )

Abstract:


Introduction: Chromatographic methods for determination of betamethasone or dexamethasone in their pharmaceutical preparations by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry were developed.

Method: Analytes were eluted from Acquity BEH C18 (50 mm×2.1 mm, 1.7 µm) column using stepwise gradient with mobile phase consisted of acetonitrile and 0.1% (v/v) acetic acid in water. Quantitation of dexamethasone and betamethasone was performed using 451>361 m/z on MRM mode.

Results: Methods were linear over the concentration range from 10 to 1500 ng mL -1 for both analyte. Limits of detection for BTM or DXM were 1 ng mL -1. The highest relative error and relative standard deviation values were not over 2%.

Conclusion: Presented methods were specific, rugged and robust, successfully applied to the analysis of their pharmaceutical preparations.

Keywords:

Dexamethasone, betamethasone, UPLC-MS/MS, tablet, ampoule, drops.

Affiliation:

Analytical Chemistry Department, Faculty of Pharmacy, Hacettepe University, Ankara, Analytical Chemistry Department, Faculty of Pharmacy, Hacettepe University, Ankara

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