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Cloud-Point Extraction Coupled with High-Performance Liquid Chromatography for the Determination and Pharmacokinetic Study of Dipyridamole in Rat Plasma

[ Vol. 13 , Issue. 5 ]

Author(s):

Jiajia Li, Yulan Li, Jingjing Liu, Min Liu and Kang Li   Pages 481 - 487 ( 7 )

Abstract:


Background: A straightforward and environment-friendly high-performance liquid chromatography/ diode array detection (HPLC/DAD) method based on cloud-point extraction (CPE) was developed for the determination of dipyridamole in rat plasma after oral administration.

Methods: The non-ionic surfactant Triton X-114 was chosen as the extract medium. Various parameters affecting the CPE efficiency were evaluated and optimized. Separation was obtained using a reversedphase Zorbax SB-C1818 column (250 × 4.6 mm, 5 µm) at 30°C with detection wavelength at 292nm

Results: Under the optimum conditions, the assay was linear over the concentration range of 0.1-20.0 µg mL-1 with the regression coefficients greater than 0.998, and the lower limit of quantification (LLOQ) was 0.1 µg mL-1. The extraction recoveries were more than 83.1%, the accuracies were within ± 9.7%, and the intra-day precision below 5.4% and inter-day precision below 12.6% in all cases.

Conclusion: The validated method is convenient, rapid and accurate for the pharmacokinetic study of dipyridamole in rat after oral administration.

Keywords:

HPLC/DAD, dipyridamole, cloud-point extraction, Triton X-114, Rat plasma, pharmacokinetics.

Affiliation:

School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong, Shenzhen Institute for Drug Control, Shenzhen, Guangdong, Shenzhen Institute for Drug Control, Shenzhen, Guangdong, Shenzhen Institute for Drug Control, Shenzhen, Guangdong, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006

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