Submit Manuscript  

Article Details


HPLC-MS Determination and Pharmacokinetic Study of 7, 8- Dihydroxyflavone, a Naturally-Occurring Neuroprotective Agent after a Single Oral Administration in Rats

[ Vol. 14 , Issue. 4 ]

Author(s):

Jianzhong Chen, Xiaomei Fu, Yixuan Dong, Mei Liu, Jian Zhang and Zhijun Liu*   Pages 343 - 348 ( 6 )

Abstract:


Background: 7, 8-dihydroxyflavone (7, 8-DHF) has been discovered to be a TrkB receptorspecific agonist and demonstrated remarkable therapeutic efficacy in vivo. These promising findings prompted the current pharmacokinetic study, in which we first established a practical LC-MS quantitative method for 7, 8-DHF then applied it in a pilot pharmacokinetic study in rats.

Methods: A simple HPLC-MS system was employed for the determination of 7, 8-DHF in rat plasma after the plasma samples were extracted with ethyl acetate by vortex-mixing. The method was thoroughly validated regarding accuracy, precision, sensitivity, linearity, selectivity, recovery, and stability.

Results: This method exhibited linearity within the concentration range of 9.024 (LLOQ) and 1128 ng/mL. The accuracy ranged from 87.9 to 105.6% of the actual values. The intra- and inter-day precisions ranged from 1.7 to 5.1% and from 2.2 to 4.4% (RSD, %). In the pharmacokinetic study, 7, 8-DHF was detectable in rat plasma for up to 24 h, with a terminal half-life of 22.8 h.

Conclusion: Overall, the results showed that this method was fully validated and successfully applied to a pilot pharmacokinetic study, which will facilitate the determination of the pharmacokinetic profile of 7, 8-DHF in future animal studies.

Keywords:

7, 8-dihydroxyflavone, HPLC-MS, neuroprotective agent, pharmacokinetics, determination, oral.

Affiliation:

School of Renewable Natural Resources, LSU Agricultural Center, Louisiana State University, Baton Rouge 70803, School of Renewable Natural Resources, LSU Agricultural Center, Louisiana State University, Baton Rouge 70803, School of Renewable Natural Resources, LSU Agricultural Center, Louisiana State University, Baton Rouge 70803, School of Renewable Natural Resources, LSU Agricultural Center, Louisiana State University, Baton Rouge 70803, School of Renewable Natural Resources, LSU Agricultural Center, Louisiana State University, Baton Rouge 70803, School of Renewable Natural Resources, LSU Agricultural Center, Louisiana State University, Baton Rouge 70803

Graphical Abstract:



Read Full-Text article