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HPLC - MS / MS Determination of Fraxetin in Rat Plasma and its Application to a Pharmacokinetic Study

[ Vol. 14 , Issue. 4 ]

Author(s):

Lei Wang, Bingjing Zheng, Guangwen Luo, Lizhu Yang, Lingtian Wang, Zhen Hu* and Zheng Xiang*   Pages 349 - 354 ( 6 )

Abstract:


Background: Fraxetin is mainly an active ingredient of Fraxini Cortex which is an important and traditional Chinese medicine for clinical applications, has heat-clearing, dispelling dampness, antitussive and anti-asthmatic effects. Fraxetin has a variety of pharmacological activities.

Method: The chromatographic separation was carried out on a Zorbax Eclipse XDB C18 column. The mobile phase consisted of acetonitrile (0.1 % formic acid) and ammonium acetate (0.1 % formic acid). Samples were prepared with protein precipitation.

Result: The method had good linearity within 10 - 2500 ng/mL. Both intra- and inter-assay precisions were acceptable and ≤ 8.5 %. Extraction recovery was 75.9 - 89.5 %, and the lower limit of quantification for fraxetin was 10 ng/mL. This proposed method was successfully used to study the pharmacokinetic and bioavailability of fraxetin after 5 and 25 mg/kg fraxetin were administered to rats via intravenous and oral routes, respectively. The half-life (t1/2) of fraxetin for i.v was (3.86 ± 0.65) h. The t1/2 of fraxetin for p.o was (4.41 ± 1.79) h. The bioavailability of fraxetin is 12.6%.

Conclusion: The results provided some useful information for the future development and clinical medication of fraxetin.

Keywords:

Fraxetin, HPLC-MS/MS, pharmacokinetic, rat, plasma, chromatography.

Affiliation:

The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325035, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, School of Medicine, Shandong University, Jinan 250012, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325035, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035

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