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A Simple and Rapid HPLC Method for the Multidetermination of Enrofloxacin, Ciprofloxacin, and Oxytetracyclin in Raw Materials and Veterinary Pharmaceutical Formulations

[ Vol. 14 , Issue. 4 ]

Author(s):

Sara da Silva Anacleto, Leila Suleimara Teixeira, Marcella Matos Cordeiro Borges, Carolina Torres Moraes, Warley de Souza Borges, Arnaldo Cesar Pereira and Keyller Bastos Borges*   Pages 397 - 404 ( 8 )

Abstract:


Background: In this work, we developed and validated a simple, rapid and accurate reverse- HPLC for the multi-determination of enrofloxacin (EFX), ciprofloxacin (CFX), and oxytetracycline (OXY) in raw materials and veterinary pharmaceutical formulations.

Method: Optimum chromatographic separations among these three analytes have been achieved within 3 min employing an Agilent Poroshell 120 EC-C18 column (100 mm × 3.0 mm, 2.7 µm) as the stationary phase using a mobile phase composted of water:acetonitrile (87: 13, v/v) plus 0.1% trifluoroacetic acid, flow rate at 3 mL min-1 and detection at 280 nm.

Result: The method resulted in excellent separation (Rs > 3) and good efficiency (theoretical plates > 5000) for all of the analytes. The method showed acceptable results in the procedure of validation according with ICH and USP guidelines with respect to selectivity, linearity, limits of detection and quantitation, accuracy, precision and robustness. Finally, the developed method can be applied in the determination of EFX, CFX and OXY in routine quality control of raw materials and veterinary pharmaceutical formulations.

Keywords:

HPLC, method validation, enrofloxacin, ciprofloxacin, oxytetracycline, veterinary.

Affiliation:

Departamento de Ciencias Naturais, Universidade Federal de Sao Joao del-Rei, Campus Dom Bosco, Praca Dom Helvecio 74, Fabricas, 36301-160, Sao Joao del-Rei, Minas Gerais, Departamento de Ciencias Naturais, Universidade Federal de Sao Joao del-Rei, Campus Dom Bosco, Praca Dom Helvecio 74, Fabricas, 36301-160, Sao Joao del-Rei, Minas Gerais, Departamento de Ciencias Naturais, Universidade Federal de Sao Joao del-Rei, Campus Dom Bosco, Praca Dom Helvecio 74, Fabricas, 36301-160, Sao Joao del-Rei, Minas Gerais, Departamento de Ciencias Exatas, Universidade Federal do Espirito Santo, 29075-910 Vitoria, ES, Departamento de Ciencias Exatas, Universidade Federal do Espirito Santo, 29075-910 Vitoria, ES, Departamento de Ciencias Naturais, Universidade Federal de Sao Joao del-Rei, Campus Dom Bosco, Praca Dom Helvecio 74, Fabricas, 36301-160, Sao Joao del-Rei, Minas Gerais, Departamento de Ciencias Naturais, Universidade Federal de Sao Joao del-Rei, Campus Dom Bosco, Praca Dom Helvecio 74, Fabricas, 36301-160, Sao Joao del-Rei, Minas Gerais

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