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Phytochemical Investigation and Quantitative Analysis of the Fatty Acids and Sterol Compounds of Seven Pharmaceutical valuable Seeds

[ Vol. 14 , Issue. 5 ]

Author(s):

Azadeh Hamedi, Maryam Sohrabpour, Mohammad M. Zarshenas and Ardalan Pasdaran*   Pages 475 - 482 ( 8 )

Abstract:


Background: This study investigates fatty acids and sterol contents of some seeds used in Asian nutrition culture to prepare functional beverages, foods or fatty acids and sterols source.

Objective: Current study presents an overview about some seeds as a valuable source for fatty acids and sterols extraction.

Methods: Gas chromatography-mass spectrometry was employed to quantify fatty acids and sterol contents and compare them to standard components. Different ash values, free amino acids, and soluble sugars were investigated. High-Performance Thin Layer Chromatography (HPTLC) was employed to detect the components. Inter- and intra-day variations, linearity of the calibration curves, and the CV of accuracy for fatty acids and sterols were generally within the acceptable ranges.

Results: The total oil content of the seeds ranged from 0.04% to 7.39%, with blue skullcap seed yielding the highest percentage of oil. The stigmasterol and β-sitosterol content of the oils ranged from 1.47 ± 0.03mg/100 g (canary grass seed oil) to 26.20 ± 0.40 mg/100 g (quince seed oil). The major monounsaturated fatty acid (MUFA)-oleic acid-was present in Quercus brantii oil, canary grass, and Crataegus aronia seeds. Linoleic acid was the most abundant polyunsaturated fatty acid (PUFA) in Entada rheedii (60.65 ±0.84%), canary grass (64.43±0.17%), and Cydonia oblonga seed oil (63.55 ±1.30%). Linolenic acid was the major fatty acid in the oil of blue skullcap (90.24 ±0.17%) and Lallemantia royleana seeds (85.18 ± 3.79%).

Conclusion: Most of the detected seeds, especially Scutellaria lateriflora and L. royleana seeds were rich sources of phytosterols and essential fatty acids.

Keywords:

Fatty acid, sterol, Scutellaria lateriflora, Entada rheedii, Lallemantia royleana, Quercus brantii.

Affiliation:

Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Student Research Committee, International branch, Shiraz University of Medical Sciences, Shiraz, Department of Traditional Pharmacy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz

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