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Eremurus spectabilis, Rich Source of Isoorientin: Isolation, Quantification and Anti-Cancer Activity on SH-SY5Y Neuroblastoma Cells

[ Vol. 14 , Issue. 6 ]

Author(s):

Esen Sezen Karaoğlan*, Gülşah Gündoğdu, Mücahit Seçme, Onur Senol, Fatma Demirkaya Miloğlu, Yavuz Dodurga and Ali Rıza Tüfekçi   Pages 578 - 585 ( 8 )

Abstract:


Background: Eremurus spectabilis BIEB. (Liliaceae) is an edible and medicinal plant in Turkey. Introduction: This study was designed to isolate and quantify isoorientin in leaves of E. spectabilis and exhibit its effect on SH-SY5Y neuroblastoma cell line.

Method: Purity and identification of isoorientin were evaluated by 1D-NMR, 2D-NMR and Q-TOF were isolated from E. spectabilis via column chromatography. An HPLC method was also developed and validated for isoorientin.

Result: Quantitative measurements indicated that contents of isoorientin in E. spectabilis leaves were 81.01 mg/g and MeOH extract were 23.75 mg/gr. All measurements were performed at 350 nm. Anticancer activity was investigated on cell culture. IC50 doses of isoorientin were detected as 250 μM at the 48th hour in SH-SY5Y cells by XTT assay. Real-time PCR analysis in SH-SY5Y cells showed that CCND1, CDK6, casp-9, Bax, ATR, Bcl-2, CHEK1 and CHEK2, expressions significantly reduced in experimental group when compared with the control group. p53, p21, caspase-3, caspase-8, Bcl-2, ATM and ERCC1 expressions increased in the experimental group when compared with the control group (P <0.05).

Conclusion: Measurements revealed that E. spectabilis contains high amount of isoorientin and can be used as a new source of this compound. In addition to this, isoorientin affects cell proliferation of neuroblastoma cells by cell cycle control and apoptosis gene expression and it could be used as a therapeutic agent, however, more studies must be performed to clarify its mechanism.

Keywords:

Eremurus spectabilis BIEB., HPLC, isoorientin, neuroblastoma, isolation, XTT.

Affiliation:

Department of Pharmaceutical Botany, Faculty of Pharmacy, Atatürk University, Erzurum, Department of Physiology, Faculty of Medicine, Atatürk University, Erzurum, Department of Medical Biology, Faculty of Medicine, Pamukkale University, Denizli, Department of Analytical Chemistry, Faculty of Pharmacy, Atatürk University, Erzurum, Department of Analytical Chemistry, Faculty of Pharmacy, Atatürk University, Erzurum, Department of Medical Biology, Faculty of Medicine, Pamukkale University, Denizli, Department of Chemistry, Faculty of Science, Çankırı Karatekin University, Çankırı



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