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Rapid Detection of AGs using Microchip Capillary Electrophoresis Contactless Conductivity Detection

[ Vol. 15 , Issue. 1 ]

Author(s):

Gangzhi Zhu, Chunjie Bao, Wenfang Liu, Xingxing Yan, Lili Liu, Jian Xiao and Chuanpin Chen*   Pages 9 - 16 ( 8 )

Abstract:


Background: In order to realize current aminoglycosides supervision in food and environment, our team improved the sensitivity and separation efficiency of the portable ITO detector, based on the technology of microchip capillary electrophoresis and contactless conductivity detection.

Experiment: Parameters (the separation voltage, buffer concentration, electrodes gap, elicitation frequency, elicitation voltage) were optimized for the detection of three aminoglycosides, gentamicin, kanamycin and streptomycin and the separation of their mixture in background electrolyte consists of 2-(N-Morpholino) ethanesulfonic acid (MES) and L-Histidine (His). The enhanced method was also applied to other types of aminoglycosides.

Results: Under optimal conditions, the monitoring of three types of aminoglycosides obtained such a sensitive response that the limits of detection of gentamicin sulfate, kanamycin sulfate and streptomycin sulfate were calculated as 3.1 µg/ml, 0.89 µg/ml and 0.96 µg/ml, at signal-to-noise ratio 3, respectively. In addition they got separated completely from each other only in 40 s. The results of other varieties of aminoglycosides including tobramycin sulfate and amikacin sulfate also met the standard.

Conclusion: We successfully proposed here an unprecedentedly portable, miniaturized and rapid microchip capillary electrophoresis contactless conductivity detection system to realize current aminoglycosides supervision in food and environment.

Keywords:

Microchip capillary electrophoresis, contactless conductivity detection, aminoglycosides, ITO-coated PET film, optimization, method validation.

Affiliation:

Haikou People's Hospital and Affiliated Haikou Hospital of Xiangya Medical School, Central South University, Haikou, Hainan 570208, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, Hunan 410013, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, Hunan 410013, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, Hunan 410013, State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, Shanghai 200237, Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, Hunan 410013, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, Hunan 410013

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