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Simultaneous Assay of ρ-Coumaric Acid and Coumarin Co-Encapsulated in Lipid-Core Nanocapsules: Validation of an LC Analytical Method

[ Vol. 15 , Issue. 2 ]

Author(s):

Ticiane Krapf Boschetti, Edilene Gadelha de Oliveira , Lucas Almeida Rigo , Karine Coradini and Ruy Carlos Ruver Beck *   Pages 145 - 151 ( 7 )

Abstract:


Background: Lipid-core nanocapsules (LNC) containing co-encapsulated ρ-coumaric acid and coumarin are under development. However, there is a lack of analytical methods to assay these bioactives in nanoformulations.

Objective: The aim of this study was to validate an LC analytical method for the simultaneous determination of ρ-coumaric acid and coumarin in lipid-core nanocapsules.

Method: The mobile phase was composed of acetonitrile:water (40:60 v/v) adjusted to pH 4 and a C-18 reversed-phase column was used. Both bioactives were detected at 275 nm. Specificity, linearity, range, precision and accuracy of the method were assessed, according to the official requirements.

Results: Nanocapsules containing ρ-coumaric and coumarin had monomodal particle size distribution, spherical-shape and Z-average size of 207 ± 2 nm. LC method was specific, linear (5 to 30 mg.mL-1), precise (RSD < 5%) and accurate (97 - 103%). It was applied to assay the content and encapsulation efficiency of the bioactive substances in LNC, which were close to 0.5 mg.mL-1 and 72%, respectively.

Conclusion: The proposed analytical method is reliable for the simultaneous assay of ρ-coumaric acid and coumarin in nanocapsules and can be further used in their development.

Keywords:

bioactives, chromatography, ρ-coumaric acid, coumarin, nanocapsules, validationbioactives, chromatography, ρ-coumaric acid, coumarin, nanocapsules, validation

Affiliation:

Departamento de Producao e Controle de Medicamentos, Programa de Pos-Graduacao em Ciencias Farmaceuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Departamento de Producao e Controle de Medicamentos, Programa de Pos-Graduacao em Ciencias Farmaceuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Departamento de Producao e Controle de Medicamentos, Programa de Pos-Graduacao em Ciencias Farmaceuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Departamento de Producao e Controle de Medicamentos, Programa de Pos-Graduacao em Ciencias Farmaceuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Departamento de Producao e Controle de Medicamentos, Programa de Pos-Graduacao em Ciencias Farmaceuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS



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