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Functionalized Multi Walled Carbon Nanotubes-Reinforced Hollow Fiber Solid/Liquid Phase Microextraction and HPLC-DAD for Determination of Phenazopyridine in Urine

[ Vol. 15 , Issue. 5 ]

Author(s):

Nabil N. Al-Hashimi*, Anas I. Awwad, Aqeel N. Al-Hashimi, Iman A. Mansi, Rand O. Shahin and Saja H. Hamed   Pages 447 - 455 ( 9 )

Abstract:


Introduction: A sensitive analytical method based on functionalized multi walled carbon nanotubes reinforced hollow fiber solid/liquid phase microextraction (F-MWCNTs-HF-SLPME) forwarded with HPLC-DAD for analyzing phenazopyridine from urine is presented.

Materials and Methods: The extraction of phenazopyridine is performed using specially designed FMWCNTs- HF-SLPME device constructed as follows: the functionalized multi walled carbon nanotubes (F-MWCNTs) were immobilized into the pores of 2.5 cm hollow fiber micro-tube using capillary forces and ultrasonication, then, the lumen of the micro-tube was filled with 1-octanol with two ends sealed. Subsequently, the device was placed into 10-mL of urine sample containing the analyte with agitation. After ending extraction, the device was removed, rinsed, sonicated in 250 µL of organic solvent and analyzed directly by the separation system.

Results and Conclusion: Different parameters affecting the performance of the developed method were optimized. The method showed good linearity with (R2) 0.999 and good repeatability with (RSDs) from 3.7 to 0.9% at analyte concentration ranged from 0.01 to 10 µg L-1 of spiked urine samples. The limit of detection/ quantitation, LODs/LOQs was 0.02/0.09 µg L-1. In comparison with reference methods, the developed method is considered as a promising microextraction technique for determination of trace phenazopyridine in human urine using a common HPLC without further cleanup procedures.

Keywords:

Functionalized multi-walled carbon nanotubes, liquid chromatography, phenazopyridine, reinforced solid/liquid phase microextraction, urine, HPLC-DAD.

Affiliation:

Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, The Hashemite University, P.O. Box 330127, Al-Zarqa 13133, Department of Chemistry, Faculty of Science, The Hashemite University, P.O. Box 150459, Al-Zarqa 13115, Department of Biochemistry, University College of Science, Osmania University, Hyderabad 500007, Department of Clinical Pharmacy and Pharmacy Practice, Faculty of Pharmaceutical Sciences, The Hashemite University, P.O. Box 330127, Al-Zarqa 13133, Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, The Hashemite University, P.O. Box 330127, Al-Zarqa 13133, Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, The Hashemite University, P.O. Box 330127, Al-Zarqa 13133

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