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RP-HPLC-UV Detection Method for the Simultaneous Determination of Econazole Nitrate, Triaminociolone Acetonide and Benzoic Acid in Ternary Mixture


Asmaa EL-Zaher, Ehab Elkady and Amaal Al-Tahami*   Pages 1 - 6 ( 6 )


Background: Reversed-phase liquid chromatography (RP-LC) method was developed for the concurrent determination of econazole nitrate, triamcinolone acetonide and benzoic acid as preservative in a ternary mixture.

Method: Separation was accomplished employing a Mediterranea C18 (150mm x 4.6mm, 5µm) column. The mobile phase used was methanol: 50 mM potassium dihydrogen phosphate buffer (pH 2.60±1), (70:30, v/v), at room temperature. The flow rate was set at 1 ml/min and UV detection was carried out at 230 nm. The Linearity, accuracy and precision of the developed method was investigated.

Results: Good separation of two drugs and preservative from each other and from excipients of dosage form with acceptable retention times (< 8 min) was obtained. Validation parameters tested including linearity, accuracy and precision were found to be satisfactory over the concentration range (10-200 μg/ml) for econazole nitrate, (1-20 μg/ml) for triamcinolone acetonide and (2-40 μg/ml) for benzoic acid.

Conclusion: The investigated method showed good separation and demonstrated to be specific, robust and accurate for the identification and quantification of the tested drugs in laboratory prepared mixtures and pharmaceutical preparations.


Econazole nitrate, Triamcinolone Acetonide, Banzoic acid, Reversed-phase liquid chromatography, Pharmaceutical preparation.


Faculty of Pharmacy, Cairo University, Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Pharmaceutical Chemistry Department, Faculty of Pharmacy, Sana`a University, Pharmaceutical Chemistry Department

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