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Development and Validation of a Specific RP-HPLC Method for Simultaneous Estimation of Anti-retroviral Drugs: Application to Nanoparticulate Formulation System

Author(s):

Nila Mary Varghese*, Venkatachalam Senthil, Sajan Jose, Cinu Thomas A and Jyoti Harindran   Pages 1 - 10 ( 10 )

Abstract:


Objective: A simple, accurate and economical Reverse Phase- High Performance Liquid Chromatographic method has been developed for the simultaneous quantitative estimation of two antiretro viral drugs, Etravirine and Elvitegravir in nanoparticulate formulations for the first time. This method is a novel analytical technique for the detection of the both the drugs concurrently, as there is no method available for their simultaneous quantification, to the best of our knowledge.

Method: Optimization and validation of the chromatographic conditions were completed according to the standard ICH guidelines. The separation was done on a C18 column (250 mm x 4.6mm, 5μm) using methanol and phosphate buffer of pH (5.6) as the mobile phase in the ratio 78:22 v/v at a flow rate of 1ml/min for a short run time of 13 min. The detection wavelength was 285nm and the column temperature was maintained at 32oC.

Results: The developed method was linear over 10 to 160

Keywords:

Etravirine, elvitegravir, HPLC, nanoformulation

Affiliation:

JSS College of Pharmacy, Pharmaceutics, Ootacamund, JSS Academy of Higher Education & Research, JSS College of Pharmacy, Pharmaceutics, Ootacamund, JSS Academy of Higher Education & Research, Centre for professional and advanced studies, Department of Pharmaceutical Sciences, Cheruvandoor, Ettumanoor, Kottayam, Kerala, Centre for professional and advanced studies, Department of Pharmaceutical Sciences, Cheruvandoor, Ettumanoor, Kottayam, Kerala, Centre for professional and advanced studies, Department of Pharmaceutical Sciences, Cheruvandoor, Ettumanoor, Kottayam, Kerala



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