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Simultaneous Determination of Methocarbamol and Paracetamol in the Presence of Three Related Substances by Ultra Performance Liquid Chromatography

[ Vol. 15 , Issue. 5 ]

Author(s):

Yanjuan Zheng, Qiushi Peng, Rui Dong*, Tingyu Chen, Yi Bao, Qianrong Peng* and Min Yang*   Pages 505 - 510 ( 6 )

Abstract:


Introduction: A rapid, and accurate Ultra Performance Liquid Chromatography (UPLC) method was developed to simultaneously analyze Methocarbamol, Paracetamol and the related substances.

Materials and Methods: Waters ACQUITY UPLC® BEH Phenyl C18 column was used in conjunction with UV detection at 225nm. Gradient elution with 0.05M, pH 6 phosphate buffer and acetonitrile flow at 0.3mL /min rate were used to separate the substances. The retention times for 4-Aminopheno, Paracetamol, Guaifenesin, Methocarbamol, and 4-Chloroacetanilide were 1.319 minute, 2.224 minute, 4.467 minute, 4.769 minute and 5.433 minute respectively. The concentration was linear in the range of 2-100 µg/ml for Methocarbamol, and 1-100 µg/mL for Paracetamol. The percentage recoveries were between 99.28±1.23% to 100.57±0.99% for Methocarbamol, and between 99.08±1.23% to 101.23±1.39% for Paracetamol.

Results and Discussion: The validated optimal protocol is robust and accurate for simultaneous analysis of Methocarbamol, Paracetamol and the related substances, applicable for bulk powder as well as pharmaceutical formulation.

Conclusion: In this paper, a highly sensitive, accurate, and precise UPLC method with UV-Vis detection was developed and validated for quality control of MET and PAR in bulk as well as in pharmaceutical preparations.

Keywords:

Methocarbamol, guaifenesin, paracetamol, 4-aminophenol, 4-chloroacetanilide, UPLC.

Affiliation:

School of Pharmaceutical Sciences, Guizhou University, Guiyang 550025, School of Pharmaceutical Sciences, Guizhou University, Guiyang 550025, Technology Center, China Tobacco Guizhou Industrial Co., Ltd., Guizhou 550009, School of Pharmaceutical Sciences, Guizhou University, Guiyang 550025, School of Pharmaceutical Sciences, Guizhou University, Guiyang 550025, Technology Center, China Tobacco Guizhou Industrial Co., Ltd., Guizhou 550009, School of Pharmaceutical Sciences, Guizhou University, Guiyang 550025

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