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An LC-MS Method for Determination of Betamethasone in Tissue Engineering of Skin and Application to Dermatopharmacokinetic Study

Author(s):

Lingjun Li, Mengli Zhang, Yuping Cao, Pengcheng Ma*, Jun Wei, Lei Tao and Kun Qian   Pages 1 - 7 ( 7 )

Abstract:


Background and Objectives: Tissue-engineering of skin is a three-dimensional skin substitute cultured in the gas-liquid interface using the immortalized keratinocytes (HaCaT cells). In this study, the preliminary metabolism of betamethasone dipropionate by tissue engineering of skin was studied and the pharmacokinetics methodology was established using betamethasone dipropionate gel as the target drug. Methods: The betamethasone dipropionate gel was applied on the tissue engineered skin after the skin was cultured. Then the medium (receiving liquid) and skin were taken on 0.25, 0.75, 1.75, 3, 5, 8, 12, 24, 36, 48 h time points. The betamethasone concentration in the medium and skin was determined by the LC-MS method. Chromatographic analysis was conducted using isocratic elution on a C18 column (150 mm

Keywords:

Betamethasone dipropionate, tissue engineering skin, determination, dermatopharmacokinetic, LC-MS

Affiliation:

Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Department of Materia Medica, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Department of Materia Medica, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Department of Materia Medica, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Department of Materia Medica, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Department of Materia Medica, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Department of Materia Medica, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Department of Materia Medica



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