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Identification of Candidate Biomarkers for HCV Leading to Hepatocellular Carcinoma Differential Stages From Serum Samples

Author(s):

Amber Afroz*, Saba Saleem, Kalsoom Sughra, Sabaz Ali Khan and Nadia Zeeshan   Pages 1 - 11 ( 11 )

Abstract:


Background: Hepatocellular carcinoma (HCC) is one of the most deadly liver malignancy found and Hepatitis C virus (HCV) is a prominent risk factor for this disease. Prognosis of HCC is poor; initiate the need of markers to discover therapeutic targets in HCC.

Introduction: Clinical staging systems of HCC composed of tumor characteristics along with liver function test are important in prognosis but they are not precise. Molecular profiling can lead to a better understanding of the physiopathology of HCC and can help in the development of novel therapeutic approaches.

Methods: 64 HCC serum samples (shifted from HCV) were graded into stage I- IV; along with +ive (3 Hepatitis C) and -ive control (2 healthy persons). Proteins were separated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and differential mRNA expression from serum samples of different HCC stages was confirmed by Real Time Polymerase Chain Reaction (qPCR).

Results: HCC serum proteins displayed differential expression of glutathione s-transferase (GST), glypican-3 (GPC3), vitronectin (VTN), and clusterin (CLU) by SDS-PAGE. GST was expressed in -ive control, while GPC3 was found in both -ive and +ive control. The qPCR analysis, display more than 0.07 fold decrease in GST in I-IV HCC stages. The highest increase in HCC stages was observed by GPC3; about 4 fold increase in I-IV stages. VTN show 1.7-3.4 fold; while CLU show 2-3.5 fold increase in four stages of HCC.

Conclusion: GPC3, VTN and CLU in combination can be good potential markers for differentiating stages (I-IV) of HCC.

Keywords:

Hepatocellular carcinoma, Vitronectin, Glypican 3, Clusterin, SDS-PAGE, Glutathione S transferase.

Affiliation:

University of Gujrat, Hafiz Hayat Campus Gujrat, Department of Biochemistry and Biotechnology, University of Gujrat, Hafiz Hayat Campus Gujrat, Department of Biochemistry and Biotechnology, University of Gujrat, Hafiz Hayat Campus Gujrat, Department of Biochemistry and Biotechnology, COMSATS, Institute of Information Technology, Abbottabad, Biotechnology Program, Department of Environmental Science, University of Gujrat, Hafiz Hayat Campus Gujrat, Department of Biochemistry and Biotechnology



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