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Pharmacokinetic Study of Deltaline in Mouse Blood Based on UPLCMS/ MS

[ Vol. 15 , Issue. 2 ]

Author(s):

Huanchun Song, Yiwei Huang, Dongqing Zhu, Shuhua Tong, Meiling Zhang, Xianqin Wang* and Xi Bao*   Pages 194 - 199 ( 6 )

Abstract:


Introduction: Deltaline, an aconitine-type alkaloid, was detected in mouse blood using an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method, and the pharmacokinetics of deltaline following intravenous administration in mice was studied.

Materials and Methods: The gelsenicine was used as the internal standard (IS). Deltaline and IS were eluted at a flow rate of 0.4 ml/min and separated on a UPLC BEH C18 column by gradient elution using acetonitrile and 10 mmol/L ammonium acetate (0.1% formic acid) as a mobile phase. The following transitions were obtained at m/z 508.2→75.0 for deltaline and m/z 327.1→107.8 for gelsenicine in multiple reactions monitoring mode. Acetonitrile was used to precipitate protein. Six mice after intravenous administration of a single dose of deltaline (1 mg/kg), 20-µL blood samples from each mouse were collected from the tail vein.

Results: The UPLC-MS/MS method was sensitive and linear (r>0.995) with a lower limit of quantitation (LLOQ) of 0.1 ng/mL over the range of 0.1-500 ng/mL. Intra- and inter-day precisions were below 13%, the accuracy range was between 88.0% and 108.2%, the recovery was higher than 90.1%, and the matrix effect was between 102.9% and 108.1%.

Conclusion: The method was sensitive, fast, specific, and has been successfully applied to a pharmacokinetic study of deltaline after intravenous administration.

Keywords:

Deltaline, pharmacokinetics, mouse, UPLC-MS/MS, blood, aconitine-type alkaloid.

Affiliation:

Department of Clinical Pharmacy, Jinhua Central Hospital, Jinhua 321000, Analytical and Testing Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Department of Clinical Pharmacy, Jinhua Central Hospital, Jinhua 321000, Analytical and Testing Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, Analytical and Testing Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000

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