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Determination of Menbutone Residues in Edible Swine Tissues Based on Solid-Phase Extraction and RP-HPLC

Author(s):

Liya Zhou, Ping Zhang, Jiangbo Liu, Xiaobo Wu, Lei Luo, Xingran Xu* and Yonghuang Luo*   Pages 1 - 9 ( 9 )

Abstract:


Background: Menbutone is widely used as a veterinary choleretic drug in many countries. There was no publicly available analysis method for the determination of menbutone residues in swine tissues. It is necessary to establish a method to control the maximum residue limit and ensure food safety of the public. Objective: The aim of this study is to establish an analytical method for the simultaneous determination of menbutone in muscle, fat, liver and kidney tissues from swine. Methods: MBT residue was extracted by acetonitrile from the tissues then purified by using a C18 solid phase extraction (SPE) cartridge and an alkaline alumina (ALA) SPE cartridge. MBT was detected by RP-HPLC and separation was achieved on a Shim-pack VP-ODS C18 HPLC column using phosphoric acid solution (0.5%, v/v) and acetonitrile (45/55, v/v) at a flow rate of 1.0 mL/min. The effluent was monitored at 235 nm, and the column temperature was set to 30 ºC. Results: MBT eluted at 6.3 min and no interfering peak nearby was observed. This linearity within the concentration range of 0.02 (LOQ) and 12 μg/mL (r2>0.9999, n=6). The accuracy ranged from 74.07 to 110.83% of the actual values. Intra and inter-day precision were within 15.11%. In the application study, MBT was detectable in continuously dosing MBT 10 μg/g/day to healthy swine for 7 days. Conclusion: The proposed method has specificity, accuracy, and sensitivity, with an excellent linear relationship that successfully applied to swine tissues.

Keywords:

Swine, Menbutone, Tissue residue, RP-HPLC, SPE, method validation

Affiliation:

College of pharmaceutical Sciences, Southwest University, Chongqing, 400715, Chongqing Municipal Agriculture School, Chongqing, 401329, College of pharmaceutical Sciences, Southwest University, Chongqing, 400715, College of pharmaceutical Sciences, Southwest University, Chongqing, 400715, College of pharmaceutical Sciences, Southwest University, Chongqing, 400715, College of pharmaceutical Sciences, Southwest University, Chongqing, 400715, College of pharmaceutical Sciences, Southwest University, Chongqing, 400715



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