Jie Ge, Jin-Wen Wang, Qi-Yan Guo and Ai-Dong Wen* Pages 1 - 6 ( 6 )
Objective: A validated liquid chromatography-tandem mass spectrometry method(LCMS/ MS) was established to simultaneously determine the concentration of triflusal and its main metabolite 2-hydroxy-4-trifluoromethyl benzoic acid(HTB) in human urine. Method: The separation was performed on a Dikma C18 column using isocratic elution with acetonitrile-4 mmol/L ammonium acetate aqueous solution containing 0.3 % formic acid water (78: 28, V/V). The method involved extraction with methanol using protein precipitation. The precursor-toproduct ion transitions with multiple reaction monitoring were m/z 247.1→161.1, 204.8→106.7and 136.9→93.0 for triflusal, HTB and salicylic acid(IS), respectively. The method showed good linear relationships over the ranges of 0.08 to 48 μg/mL and0.5 to 50 μg/mL. Results: It was the first time that a urinary excretion study of triflusal capsule as oral. The cumulative urinary recovery showed 8.5% and 2.7% for triflusal and HTB, respectively. Conclusion: This method was successfully used for evaluating the pharmacokinetic properties of triflusal and HTB in urine in Chinese healthy subjects.
triflusal, HTB, method, determine, analysis, urine
Department of Pharmacy, Xijing hospital, Xi`an, Shaanxi710032, Department of Pharmacy, Xijing hospital, Xi`an, Shaanxi710032, Department of Pharmacy, Xijing hospital, Xi`an, Shaanxi710032, Department of Pharmacy, Xijing hospital, Xi`an, Shaanxi710032