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Comparison Study on the Contents of Eight Flavonoids in three Different Processed Products of Scutellariae Radix using Ultra-high Performance Liquid Chromatography Coupled With Triple-Quadrupole Mass Spectrometry

Author(s):

Yuedong Yang, Hao Zhi, Baofei Yan, Yi Tian, Jianping Shen, Xingyu Zhu* and Zhipeng Li*   Pages 1 - 8 ( 8 )

Abstract:


Background: The simultaneous determination of multiple components in a sample is an important factor in the quality control of traditional Chinese medicines and can give an indication of potential clinical applications.

Introduction: A rapid and sensitive method for the simultaneous quantitative analysis of eight bioactive flavonoid constituents from Scutellariae Radix using ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry.

Method: The separation was performed on a Waters Acquity UPLC C18 column (2.1 mm×100 mm, 1.7 μm), under optimized mass spectrometry conditions, with a flow rate of 0.3 mL/min. The column temperature was maintained at 35℃ and the injection volume was 3 μL.

Result: The method showed a good linear relationship of each component, all R2 values were above 0.9990 in the experiment. The RSDs of the precision test, repeatability test, stability test and recovery test were all no more than 2.86 %. We found that the total percentage amounts of the eight flavonoids were 22.19%, 18.63% and 10.86% in Raw Scutellariae Radix (RSR), Wine Scutellaria Radix (WSR) and Scutellaria Radix Charcoal (SRC) respectively.

Conclusion: The method was successfully applied to the simultaneous determination of the eight bioactive flavonoids of Raw Scutellariae Radix, Wine Scutellaria Radix and Scutellaria Radix Charcoal.

Keywords:

Scutellariae Radix, Processing, UHPLC-TQ-MS/MS, Quantification, Flavonoids, Quality control

Affiliation:

Nanjing University of Chinese Medicine, Nanjing, PR, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, Jiangsu Health Vocational College, Nanjing, Nanjing University of Chinese Medicine, Nanjing, PR, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, Jiangsu College of Nursing, Huaian, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, PR



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