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The Application of TLC and Densitometry for Quantitative Determination of Meloxicam in Tablets

Author(s):

Wioletta Parys, Katarzyna Bober, Alina Pyka-Pająk* and Małgorzata Dołowy   Pages 1 - 10 ( 10 )

Abstract:


Background: Meloxicam is as a non-steroidal anti-inflammatory drug that indicates a strong anti-inflammatory, analgesic and antipyretic activity. It is used in the treatment of osteoarthritis arthritis, osteoarthritis and rheumatoid arthritis in form of various pharmaceutical preparations.

Objective: The aim of the work was elaboration of chromatographic conditions enabling the complete separation of impurities A and B from meloxicam and also its quantitative determination in tablets with use of TLC combined with densitometry as well as the comparison of the method proposed with that described in literature by Starek and Krzek.

Method: The mixture of ethyl acetate: toluene: n-butylamine (2:2:1, v/v/v) was used as a mobile phase. Determination of meloxicam was performed on silica gel and aluminium oxide plates. Chromatographic conditions presented in this work are better than those described by Starek and Krzek.

Results: Linearity of the method for both type of plates was in the range from 1.0 to 5.0 µg/spot. Limit of quantification for silica gel plates was 0.18 µg/spot, while for aluminium oxide plates was 0.26 µg/spot. Limit of detection has been also specified, 0.06 µg/spot for silica gel plates and 0.08 µg/spot for aluminium oxide plates. Average amount of meloxicam in tablets obtained on silica gel plates was 100.4%, and on aluminium oxide plates was 100.3%.

Conclusion: The developed method of determination of meloxicam using thin layer chromatography combined with densitometry turned out to be accurate, precise and specific. It can be successfully applied in quality control of meloxicam.

Keywords:

chromatography, TLC, densitometry, meloxicam, validation, nonsteroidal anti-inflammatory drugs

Affiliation:

Department of Analytical Chemistry, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia in Katowice, Katowice, Department of Analytical Chemistry, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia in Katowice, Katowice, Department of Analytical Chemistry, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia in Katowice, Katowice, Department of Analytical Chemistry, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia in Katowice, Katowice



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