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Advanced High-Coverage Targeted Metabolomics Method (SWATHtoMRM) for Exploring the Relationship of Follicular Fluid Components with Age

Author(s):

Jingyan Song, Tianqi Wang, Jiayin Guo, Ying Guo, Xiaoming Wang, Yi Yang, Kaiyue Xu, Yuanhong Sa, Lihua Yuan, Huaying Jiang and Zhengao Sun*   Pages 1 - 11 ( 11 )

Abstract:


Background: The complexity of follicular fluid metabolome presents a huge challenge for qualitative and quantitative metabolite profiling and discovering the comprehensive biomarkers.

Objective: In order to address this challenge, novel SWATHtoMRM metabolomics method was used for providing the broad coverage and excellent quantitative capability to discovery the human follicular fluid metabolites related to age and evaluate their relationship with pregnancy outcome and oocyte senescence.

Method: The patients were divided into four groups according to age, including group A (28 cases, 21–27 years old), group B (42 cases, 28–34 years old), group C (31 cases, 35–41 years old), and group D (24 cases, 42–48 years old). Follicular fluid samples from 125 IVF patients were analyzed. The differential ions among the four groups were identified by principal components analysis according to accurate mass, isotope ratio, and mass spectrometric fragmentation patterns. Then, the differential metabolic pathways were further identified by a KEGG cluster analysis.

Results: A total of 18 metabolites in the follicular fluid differed among the four groups, including amino acids, lipids, hormones, and vitamins. A total of 15 metabolites, including 6-oxohexanoate, phenylalanine, proline, hexadecanoic acid, linoleate, arachidonate, oleic acid, docosahexaenoic acid, LysoPC(16:1), LysoPC(20:45), LysoPC (20:3), 25-hydroxyvitamin D3, 5-dehydroepisterol, 27-hydroxycholesterol, and 5beta-cholestane-3alpha,7alpha,12alpha,23,25-pentol, were down-regulated with age and 3 metabolites, including LysoPC(18:3), LysoPC(18:1), and 13,14-dihydroretinol, were up-regulated with age.

Conclusion: Our study provides useful information for revealing the relationship between age and female reproductive capability.

Keywords:

Follicular fluid, metabolomics, age, reproductive function, SWATHtoMRM, UPLC-QTOF

Affiliation:

Reproductive and Genetic Center of Integrated Traditional and Western Medicine, the Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Department of Gynecology and Obstetrics of Traditional Chinese Medicine, the First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, Reproductive and Genetic Center of Integrated Traditional and Western Medicine, the Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Department of Gynecology and Obstetrics of Traditional Chinese Medicine, the First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan, Department of Gynecology and Obstetrics of Traditional Chinese Medicine, the First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan, Department of Gynecology and Obstetrics of Traditional Chinese Medicine, the First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan, Department of Gynecology and Obstetrics of Traditional Chinese Medicine, the First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan, Department of Gynecology and Obstetrics of Traditional Chinese Medicine, the First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan, Department of Gynecology and Obstetrics of Traditional Chinese Medicine, the First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan, Reproductive and Genetic Center of Integrated Traditional and Western Medicine, the Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan



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