Submit Manuscript  

Article Details


UPLC-MS/MS Method for Determination of Khasianine in Mouse Blood: Application for Its Pharmacokinetic Study

Author(s):

Lianguo Chen, Qinghua Weng, Yijing Lin, Xiaojie Lu, Zuoquan Zhong, Jianhua Xiong and Xianqin Wang*   Pages 1 - 7 ( 7 )

Abstract:


Background: The aim of this study was to determine the concentrations of khasianine in mouse whole blood sample and its application for the pharmacokinetics by a rapid, selective and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method.

Methods: The blood samples were preprocessed by one-step protein precipitation with acetonitrile. The study was performed on an ACQUITY I-Class UPLC system with a UPLC BEH column. Lannaconitine (internal standard, IS) and khasianine were gradient eluted by a mixture of acetonitrile and water with 0.1% formic acid at a flow of 0.4 mL/min. The mass spectrometer was equipped with an electrospray ionization (ESI) source in positive mode. The quantitative detection performed in a multiple reaction monitoring mode at transitions m/z 722.4→70.7 for khasianine and m/z 585.3→119.9 for the corresponding IS.

Results: The calibration curve was of good linearity ranged from 0.5 to 1000 ng/mL (r > 0.995). The lower limit of detection (LLOD) and lower limit of quantitation (LLOQ) were 0.2 and 0.5 ng/mL, respectively. The inter-day and intra-day precision (RSD%) were both less than 14%, and the accuracy ranged from 86.6% to 108.3%. The matrix effects were between 98.0% and 103.7%, and the average recovery was better than 67.4%.

Conclusion: This assay established a sensitive, rapid, selective UPLC-MS/MS method which was successfully used for the pharmacokinetic study of khasianine in mouse blood, and the absolute availability of khasianine was 0.78% exhibited a poor oral absorption.

Keywords:

khasianine, mouse, UPLC-MS/MS, determination, pharmacokinetics, bioavailability

Affiliation:

Department of pharmacy, Wenzhou People`s Hospital, Wenzhou 325000, Department of pharmacy, Wenzhou People`s Hospital, Wenzhou 325000, Analytical and testing Centre, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, Analytical and testing Centre, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, Analytical and testing Centre, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, Department of pharmacy, Wenzhou People`s Hospital, Wenzhou 325000, Analytical and testing Centre, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035



Read Full-Text article