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Pharmacokinetics of Benzoic Acid, 4-[[(2-Hydroxyethyl)Amino]Carbonyl]- , Methyl Ester from Portulaca Oleracea L. in Rats after Intravenous and Oral Administrations Using UHPLC-ESI-Q-TOF/MS

Author(s):

Haoran Xu, Zheming Ying, Lina Wang, Wenjie Zhang, Xixiang Ying* and Guanlin Yang*   Pages 1 - 7 ( 7 )

Abstract:


Objective: The aim of this study is to investigate the pharmacokinetics of benzoic acid, 4- [[(2-hydroxyethyl)amino]carbonyl], methyl ester in rats after intravenous and oral administrations at doses of 3 mL/kg.

Methods: A rapid, high selective ultra-high performance liquid chromatographic electrospray quadrupole- time of flight mass spectrometry (UHPLC-ESI-Q-TOF/MS) method was applied to investigate the pharmacokinetics of benzoic acid, 4-[[(2-hydroxyethyl)amino]carbonyl]-, methyl ester with pcoumaric acid as internal standard (IS) in rats after intravenously and orally dosed.

Results: The pharmacokinetic data of benzoic acid, 4-[[(2-hydroxyethyl)amino]carbonyl]-, methyl ester was analyzed in the two-compartment open model. The main pharmacokinetic parameters were, respectively, 36.474 μg·h/mL, 12.59 μg·h/mL (AUC0), and T1/2α was 0.14 h, 0.359 h; T1/2β was 3.046 h, 5.646 h after intravenous and oral administrations.

Conclusion: Benzoic acid, 4-[[(2-hydroxyethyl)amino] carbonyl]-, methyl ester was rapidly distributed in rat’s plasma with the absolute bioavailability of 34.5%.

Keywords:

Portulaca oleracea L., Benzoic acid, 4-[[(2-hydroxyethyl)amino]carbonyl]-, methyl ester, Pharmacokinetics, Ultra-high performance liquid chromatography electrospray quadrupole-time of flight mass spectrometry, Rat.

Affiliation:

School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, P.R., School of The First Clinic, Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, P.R., School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, P.R., School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, P.R., School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, P.R., School of The First Clinic, Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, P.R.



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