Submit Manuscript  

Article Details


An Easy Efficient Method of Veterinary Drug Residue Analysis in Raw Milk by RP-HPLC-UV with Application to Raw Milk

Author(s):

Lekweiri Haiba Legrae, Mohamed Fadel Deida, Bah Mohamed Lemine Abdellahi, Mohamed Brahim Elkory, Jalloul Bouajila* and Ibrahima Ndiaye   Pages 1 - 7 ( 7 )

Abstract:


Background: This study reports an easy method of veterinary drug investigation in raw milk, based on QuECHERS extraction followed by RP-HPLC-UV analysis. Use of this benchtop system was motivated by its availability and moderate cost relatively to other sophisticated methods such as LC-MS which are more performant.

Methods: This developed method has been optimized and then after validated according to EU legislation, demonstrating good linearity with R²>0.997, acceptable peak resolution within short time (˂9.5 min) and a good recovery of analyzed drugs (OXY, ALZ and IVR, respectively 87.08, 99.02 and 92.01%). Additionally we applied the method to analysis of cow milk, collected in Nouakchott, capital of Mauritania.

Results: Obtained results indicates a mixed level of drug use according to targeted molecules. In 42% of sampled farms the anti-parasitics IVR and ALZ were detected whereas the antibiotic OXY was detected in 50%.

Conclusion: This investigation shows that 17% of sampled farms exceed European standards for IVR drug.

Keywords:

Veterinary drug residue, oxytetracycline, ivermectin, albendazole, QuECHERS method, sahel region

Affiliation:

Université de Toulouse, Faculté de pharmacie de Toulouse, Laboratoire des IMRCP UMR CNRS 5623, Université Paul-Sabatier, 118 route de Narbonne, F-31062 Toulouse, Unité de Recherche de Chimie Moléculaire et Environnement, Faculté des Sciences et Techniques, Université moderne de Nouakchott, Unité de Recherche de Chimie Moléculaire et Environnement, Faculté des Sciences et Techniques, Université moderne de Nouakchott, Laboratoire de contrôle qualité des eaux et aliments, Institut National de Recherches en Santé Publique (INRSP), Université de Toulouse, Faculté de pharmacie de Toulouse, Laboratoire des IMRCP UMR CNRS 5623, Université Paul-Sabatier, 118 route de Narbonne, F-31062 Toulouse, Groupe de Recherche sur les Substances Bioactives, Faculté des Sciences et Techniques, Université Cheikh Anta Diop de Dakar



Read Full-Text article