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Investigation on the Metabolism of Curcumin and Baicalein in Zebrafish by Liquid Chromatography-Tandem Mass Spectrometry Analysis

Author(s):

Shi-Jun Yin, Ya-Li Wang, Hua Chen, Guang Hu*, Guo-Can Zheng and Feng-Qing Yang*   Pages 1 - 7 ( 7 )

Abstract:


Background: Curcumin (CUR) and baicalein (BAI) are the main active ingredients in Curcuma longa and Scutellaria baicalensis, which were used together in Jiang-Qin-Si-Wu decoction to treat gynecological diseases. On the other hand, zebrafish as metabolic model has become more and more popular, so the metabolism of CUR and BAI in zebrafish was investigated in the present study.

Methods: Zebrafish embryos after hatching 48 hours were divided into four experimental groups. The blank group was exposed in 1 mL ultra-pure water. Three drug-treated groups were exposed in CUR (8 μM, 1 mL), BAI (8 μM, 1 mL), CUR and BAI (8 μM, 2 mL), respectively. After homogenization, they were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The structure of the metabolites was determined by comparing their corresponding mass spectra with those of relevant literatures. According to the change of metabolite content, the metabolic effect of curcumin and baicalein was explored.

Results: Five and six metabolites of CUR and BAI in zebrafish were identified by LC-MS/MS, respectively. Their metabolic pathways in zebrafish were glucuronidation and sulfation. Reduction or methylation reactions also occurred for CUR and BAI, respectively. In addition, after combined exposure of both drugs, CUR reduced the BAI glucuronide metabolites and could inhibit the metabolism of BAI in zebrafish, which is consistent with mammalian metabolism.

Conclusion: Using LC-MS/MS analysis, zebrafish is a feasible model for drug metabolism study. The results of metabolic study indicated that CUR might affect the therapeutic effect of BAI.

Keywords:

Zebrafish, LC-MS/MS, Curcumin, Baicalein, Metabolic pathway, Metabolic effect

Affiliation:

School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, Analytical and Testing Center, Chongqing University, Chongqing 401331, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331



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