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Characterization of 10 Biogenic Amines in Male Silkworm Moth by HPLC with Precolumn Derivatization

Author(s):

Mingqin Fan, Yun Ai, Wenjie Zhao, Yanni Sun, Jianli Liu and Ning Zhang   Pages 1 - 7 ( 7 )

Abstract:


Background: Biogenic amines (BAs) are biologically active nitrogenous organic compounds of low molecular weight, which are frequently found in a wide variety of foods, beverages and herbs due to their toxic potential in humans. Male silkworm moth (MSM), a Traditional Chinese Medicine (TCM), has been exploited and utilized as nutritious liquor based on its traditional effects in Chinese community.

Method: An HPLC with Dns-Cl derivatization method for characterizing overall BAs in MSM and providing data for further evaluating its activities and safety profiles. The method has acceptable sensitivity, precision, accuracy, selectivity and recovery, and was successfully applied to the determination of the BAs contents in MSM for the first time.

Results:In the analysis of 10 batches of MSM samples, 5-HT and DA were not found in detectable concentrations in any samples, and the most abundant amine found was PUT. The mean values of TRY, PEA, PUT, CAD, HIS, TYR, SPD, and SPM determined in the samples were found to be 34.7 mg/ kg, 16.1 mg/ kg, 218.3 mg/ kg, 37.9 mg/ kg, 12.1 mg/ kg, 18.2 mg/ kg, 4.5mg/ kg, and 0.9 mg/ kg, respectively.

Conclusion:The contents of BAs in ten batches of MSM were below the maximum recommended limits, and MSM can be used safely.

Keywords:

Biogenic amines, Male silkworm moth, Derivatization, HPLC, Dns-Cl, safety profiles

Affiliation:

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Science, Northwest University, 229 Taibaibei Road, Xi`an 710069, Xi`an Institute for Food and Drug Control, Xi’an 710054, Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Science, Northwest University, 229 Taibaibei Road, Xi`an 710069, Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Science, Northwest University, 229 Taibaibei Road, Xi`an 710069, Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Science, Northwest University, 229 Taibaibei Road, Xi`an 710069, Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Science, Northwest University, 229 Taibaibei Road, Xi`an 710069



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