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Trace Determination of Tamoxifen using Optimized Solvent Bar Microextraction and HPLC-UV


Persia Behbahani, Nahid Ghasemi, Mahnaz Qomi* and Kambiz Tahvildari   Pages 1 - 6 ( 6 )


Background: Tamoxifen (Soltamox) is an antineoplastic agent and an estrogen receptor antagonist with the indications for breast cancer, with severe side effects such as hot flashes, vaginal discharge, etc. Dose monitoring is a necessity for optimum treatment, free of severe adverse effects.

Method: The solvent bar microextraction method (SBME) was used for preconcentration and microextraction coupled with High-performance liquid chromatography-ultraviolet (HPLC-UV) analysis of tamoxifen in this study.

Results: The limit of detection and limit of quantification were 13.3 and 40 µgL-1, respectively. The linear range was between 40 and 10000 µgL-1 with a correlation coefficient of 0.999. The enrichment factor was 169 and the relative standard deviation within-day and between-day were 3.6 and 4.0, respectively.

Conclusion: The use of trend and sensitive SBME method coupled with HPLC-UV analysis for detection of tamoxifen at trace level was successful, offering a desirable preconcentration factor and cost-effective and green set up for determining the rate of elimination in cancer patients and purification of wastewater.


Tamoxifen, solvent bar microextraction, HPLC-UV, chemometrics, real sample


Department of Chemistry, Sciences Faculty, Arak Branch, Islamic Azad University, Arak, Department of Chemistry, Sciences Faculty, Arak Branch, Islamic Azad University, Arak, Active Pharmaceutical Ingredients Research Center, Medical Sciences Branch, Islamic Azad University, Tehran, Faculty of Chemistry, Islamic Azad University, North Tehran Branch, Tehran

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