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A Versatile Stability-Indicating Liquid Chromatographic Method for the Simultaneous Determination of Atenolol, Hydrochlorothiazide and Chlorothalidone

Author(s):

Ehab Farouk Elkady, Marwa Ahmed Fouad and Abdulgabar A. Ezzy Faquih*   Pages 1 - 14 ( 14 )

Abstract:


Background: Atenolol is a selective beta 1 blocker that can be used alone or in combination with hydrochlorothiazide or with chlorothalidone for treatment hypertension and prevention from heart attack . Objective: The main target of this work was to improve modern, easy, accurate and selective liquid chromatographic method (RP-HPLC) for the determination of these drugs in the presence of their degradation products . These methods can be used as analytical gadgets in quality control laboratories for routine examination.

Methods: In this method, the separation was accomplished through an Inertsil® ODS -3V C18 column (250 mm x 4.6 mm, 5 µ m) the mobile phase used was 25 mM aqueous potassium dihydrogen orthophosphate solution adjusted to pH 6.8 by using 0.1M sodium hydroxide and acetonitrile (77 : 23, v/v), the flow rate used was 1 ml/min and detection at 235 nm using UV.

Results: All peaks were sharp and well separated, the retention times were atenolol degradation (ATN Deg.) 2.311 min, atenolol (ATN) 2.580 min, hydrochlorothiazide degradation (HCT Deg.) 5.890 min, hydrochlorothiazide (HCT) 7.016 min, chlorothalidone degradation CTD Deg 8.018 min and chlorothalidone (CTD) 14.972 min.. Linearity was obtained and the range of concentrations were (20- 160 µg/ml) for atenolol, (10-80 µg/ml) for hydrochlorothiazide and (10 -80 µg/ml) for chlorothalidone. According to ICH guidelines, method validation was accomplished, these methods include linearity, accuracy, selectivity, precision and robustness.

Conclusion: The optimized method demonstrated to be specific, robust and accurate for the quality control of the cited drugs in pharmaceutical dosage forms.

Keywords:

Atenolol, Hydrochlorothiazide, Chlorothalidone, Reversed phase liquid chromatography, Pharmaceutical preparations

Affiliation:

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., Cairo 11562, Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., Cairo 11562, Pharmaceutical Chemistry Department, Hodeida University, Faculty of Clinical Pharmacy, Hodeida



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