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Determination of Pyridostigmine Bromide in Presence of its Related Impurities by Four Modified Classical Least Square Based Models: A Comparative Study

Author(s):

Fatma F. Abdallah*, Eglal A. Abdelaleem, Aml A. Emam and Ibrahim A. Naguib   Pages 1 - 8 ( 8 )

Abstract:


Introduction: Novel manipulations of the well-known classical least squares multivariate calibration model were explained in details as a comparative analytical study in the introduced research work. In addition to application of plain classical least squares model, two preprocessing steps were tried, where prior to modeling with classical least squares, first derivatization and orthogonal projection to latent structures were applied to produce two novel manipulations of classical least square based model. Moreover, spectral residual augmented classical least squares model is included in the presented comparative study.

Quantitative determination of pyidostigmine bromide in presence of its two related substances; impurity A and impurity B was considered as a case study for constructing the comparison.

Method: 3 factor 4 level design was implemented constructing a training set of 16 mixtures with different concentrations of the studied components. To investigate the predictive ability of the studied models; a test set consisting of 9 mixtures was constructed.

Results: The key performance indicator of this comparative study was the root mean square error of prediction for the independent test set mixtures, where it was found 1.367 when classical least squares applied with no preprocessing method, 1.352 when first derivative data was implemented, 0.2100 when orthogonal projection to latent structures preprocessing method was applied and 0.2747 when spectral residual augmented classical least squares was performed.

Conclusion: Coupling of classical least squares model with orthogonal projection to latent structures preprocessing method produced significant improvement of the predictive ability of it.

Keywords:

First derivative, Orthogonal projection to latent structures, Classical least squre, Spectral residual augmented classical least squares, Pyridostigmine bromide.

Affiliation:

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmad Hegazy St., 62514, Beni-Suef, Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmad Hegazy St., 62514, Beni-Suef, Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmad Hegazy St., 62514, Beni-Suef, Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmad Hegazy St., 62514, Beni-Suef



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