Lovekesh Mehta*, Parul Grover, Tanveer Naved and Debaraj Mukherjee Pages 1 - 8 ( 8 )
To develop effective and safe drugs and to take them to market in short period of time is the mission of pharmaceutical research companies. A selection of few of the lead compounds are done for the evaluation of safety and their ADMET (absorption, distribution, metabolism, excretion and toxicology) properties are tested in in-vitro (test systems), in-vivo (living organisms) and in-silico (computational methods). From initial stages to final stages of modern drug discovery process, the vital tool for detecting & characterizing metabolites is MS (Mass spectrometry) hyphenated with other techniques. The methods used for generation of metabolites are in-vitro techniques and cell lines (containing expressing drug metabolizing enzymes and heterologous genes). The use of HPLC-MS/UPLC-MS and high resolution MS, enables the in depth metabolite detection and profiling studies and it may also be likely to identify and characterize the site and types of biotransformation.
biotransformation, regioselective, preformulation, ICH, forced degradation studies
Research Scientist, Teva API India Pvt. Ltd., Greater Noida, Assistant Professor, KIET School of Pharmacy, KIET Institute, Ghaziabad, Joint Head, Amity Institute of Pharmacy, Amity University, Noida, Scientist, Indian Institute of Integrative Medicine, Jammu