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Pharmacokinetic Differences of Grape Seed Procyanidins According to the Gavage Administration Between Normal Rats and Alzheimer's Disease Rats

Author(s):

Xinhui Cheng, Jingying Zhang, Huiting Jing, Yu Qi , Tingxu Yan, Bo Wu, Yiyang Du, Feng Xiao* and Ying Jia *   Pages 1 - 10 ( 10 )

Abstract:


Background: Grape Seed Procyanidins (GSP) are a kind of natural polyphenols with strong antioxidant capacity. Studies have shown that GSP have a significant effect in the alleviation of Alzheimer's Disease (AD).

Objective: In order to investigate whether there is a pharmacokinetics difference in GSP between normal and AD rats, a rapid UPLC-MS/MS method for detecting the content of it in plasma samples was established, and we analyzed the plasma concentrations of procyanidin B2, procyanidin B3, catechin and epicatechin in normal and AD rats over time to determine the plasma concentration of GSP.

Methods: We used 400 μL of methanol for the protein precipitation solvent in the plasma treatment. The chromatographic separation was performed on a C18 column at 20 ℃. The mobile phase was a gradient of 0.1% formic acid in water and methanol, time was 15 min.

Results: In this study, the plasma concentrations of procyanidin B2, procyanidin B3, catechin and epicatechin in AD rats were significantly higher than those in normal rats (P < 0.05). And the content of epicatechin was the highest compontent than catechin, procyanidin B2 and procyanidin B3 after administration of GSP.

Conclusion: We found that the absorptions of these analytes in AD group is better than in normal group, which provides an analytical basis for the treatment of AD with procyanidins.

Keywords:

Grape seed procyanidins, UPLC-MS/MS, pharmacokinetics, Alzheimer's disease, plasma concentration, gavage administration

Affiliation:

School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, School of Functional Food and Wine, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR , School of Functional Food and Wine, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR , School of Functional Food and Wine, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR , School of Functional Food and Wine, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR , School of Functional Food and Wine, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR



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