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Pharmacokinetics of Picroside I, II, III, IV in Rat Plasma by UPLCMS/ MS

Author(s):

Haili Xie, Xiaojie Lu, Weiqiang Jin, Hua Zhou, Dongxin Chen, Xianqin Wang* and Yunfang Zhou*   Pages 1 - 8 ( 8 )

Abstract:


Background: Modern pharmacological studies show that rhizoma coptidis has protective effects on liver, gallbladder, kidney, cerebral ischemia-reperfusion, local hypoxia injury, anti-inflammatory, bone injury, nerve cells and myocardial cells. The effective components have been isolated from picroside I, II, III and IV.

Introduction: A selective and sensitive ultra-performance liquid chromatography electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) method was developed for the simultaneous quantitative determination of picroside I, II, III and IV in rat plasma to aid the pharmacokinetics studies.

Method: Sprague-Dawley (SD) rats were orally administered with 10 mg/kg, intravenously injected with 1 mg/kg for the mixture of picroside I, II, III and IV. The biological samples were collected at 0.083 3 h, 0.25 h, 1 h, 2 h, 4 h, 6 h, 8 h, 12 h, 24 h. An UPLC BEH C18 column (2.1 mm×50 mm,1.7 μm) was used for chromatographic separation with the mobile phase consisted of acetonitrile and 0.1% formic acid by gradient elution, the flow rate was 0.4 mL/min. Multiple reaction monitoring (MRM) transitions were m/z 491.1→147.1 for picroside I , m/z 511.1→234.9 for picroside II, m/z 537.3→174.8 for picroside III and m/z 507.3→163.1 for picroside IV in negative ion mode.

Result: The inter-day precision was less than 13%, the intra-day precision was less than 15%. The accuracy ranged from 89.4% to 111.1%. Recovery was higher than 79.1%, and the matrix effect ranged from 96.2% to 109.0%.

Conclusion: The sensitive, rapid and selective UPLC-MS/MS method can be applied to the pharmacokinetic study of picroside I, II, III and IV in rats.

Keywords:

Picroside, pharmacokinetics, bioavailability, rat, UPLC-MS/MS, plasma

Affiliation:

Department of pharmacy, Lihuili hospital, Ningbo Medical Center, Ningbo 315040, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, Department of pharmacy, Lihuili hospital, Ningbo Medical Center, Ningbo 315040, Department of pharmacy, Lihuili hospital, Ningbo Medical Center, Ningbo 315040, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, Department of Pharmacy, The People's Hospital of Lishui, Lishui 323000



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