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Chiral Separation of Oxomemazine Enantiomers by HPLC Technique and Enantiomeric Separation Mechanism via Docking Studies

Author(s):

Prachi Raikar*, B. M. Gurupadayya, Subhankar P. Mandal, Maruthi R and Prabitha Prabhakar   Pages 1 - 9 ( 9 )

Abstract:


Background: A normal phase- High Performance Liquid Chromatographic (HPLC) method was developed for the enantioseparation of Oxomemazine.

Method: Separation of enantiomers was attained on Amylose Tris (5-chloro-2-methylphenylcarbamate) using n-hexane: Iso-propyl Alcohol (IPA): Diethylamine (DEA) (60: 40: 0.1) as the mobile phase and the peaks were observed at 227nm using PDA detector. The run time of the analysis was set to 30 min.

Results: Linearity was found in the range 10-50 µgmL-1. The enantiomers were separated at retention times 16.87 min and 21.37 min.

Conclusion: The developed method was validated as per the ICH guidelines, thus proving the method to be selective, precise and showing linear response of Oxomemazine peak areas. Additionally, the method of chiral separation being understood by docking simulation study. The method was appropriate for analysis of Oxomemazine in the pure form and its formulation.

Keywords:

Analytical chemistry, Chirality, Chromatography, High Performance Liquid Chromatography, Liquid Chromatography

Affiliation:

Dept. of Pharmaceutical Chemistry, JSS College of Pharmacy, Mysuru- Karnataka, Dept. of Pharmaceutical Chemistry, JSS College of Pharmacy, Mysuru- Karnataka, Dept. of Pharmaceutical Chemistry, JSS College of Pharmacy, Mysuru- Karnataka, Dept. of Pharmaceutical Chemistry, JSS College of Pharmacy, Mysuru- Karnataka, Dept. of Pharmaceutical Chemistry, JSS College of Pharmacy, Mysuru- Karnataka



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