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Simultaneous Determination of Seven Active Components in Desheng Pills by High-Performance Liquid Chromatography

Author(s):

Yang Xu, Fang Wang*, Huiwei Bao, Huailei Yang, Baiyu Shan, Kuo Fang and Mingyu Li   Pages 1 - 10 ( 10 )

Abstract:


Background: Desheng pills(DSP) consists of six traditional Chinese medicine. This preparation has the effects of nourishing blood, eliminating stasis, smoothing liver and regulating menstruation, which can be used to treat menoxenia and dysmenorrhea caused by qi stagnation and blood stasis.

Objective: In this paper, an accurate and sensitive high-performance liquid chromatography–diode array detector (HPLC-DAD) method was developed and validated for simultaneous determination of seven active components(gallic acid, paeoniflorin, costunolide, dehydrocostuslactone, rutin, leonurine hydrochloride and ferulic acid) in the traditional Chinese formula-Desheng pills.

Methods: The seven analytes were separated on Agilent ZORBAX SB-C18 column(250mm× 4.6mm, 5μm) maintained at the temperature of 30℃. Gradient elution was performed with the mobile phase of methanol(A)-0.1% phosphoric acid solution(B) at the flow rate of 1.0mL·min-1. The analysis was carried out at the wavelength of 225 nm, 256 nm, 277 nm and 320 nm with an injection volume of 10 μL.

Results: The measured seven components showed good linear relationships within their own concentration ranges along with coefficients of determination ≥0.9996. The limits of detection and quantitation of all analytes were in the range of 0.19-13.51 μg/mL and 0.59-40.93 μg/mL, respectively. Average recoveries ranged from 98.82% to 102.01% with RSDs of 1.47%-1.99%. The content of tested components were in the range of 0.053-0.421 mg/g.

Conclusion: The proposed method was found to be sensitive, accurate and reproducible, which provided an effective quantitative analytical method for quality control of Desheng pills.

Keywords:

High-Performance Liquid Chromatography(HPLC), Diode Array Detector(DAD), Desheng Pills(DSP), Active Components, Simultaneous Determination, Quality Control

Affiliation:

School of Basic Medical Sciences, Jilin University, Changchun, School of Basic Medical Sciences, Jilin University, Changchun, College of pharmacy, Changchun University of Chinese Medicine, Changchun, Plant Chemistry Laboratory, Chinese Institute of Jilin Ginseng, Changchun, Baicheng Institute for Food and Drug Control, Baicheng, College of Pharmaceutical and Food, Changchun Medical College, Changchun, College of pharmacy, Changchun University of Chinese Medicine, Changchun



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