Wenhao Sun, Xiajuan Jiang, Xianqin Wang* and Xi Bao Pages 1 - 7 ( 7 )
Introduction: In this study, a precise, rapid and accurate ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the quantification of zhebeirine in mouse blood was developed, and pharmacokinetics of zhebeirine was studied for the first time after intravenous and oral administration.
Method: The mobile phase consisted of acetonitrile-0.1% formic acid, with a flow rate at 0.4 mL/m during 4 min run time. MRM modes of m/z 414.5→81.0 for zhebeirineand m/z 430.2→412.2 for 3-dehydroverticine (internal standard) were utilized to quantitative analysis. Protein mouse blood was directly precipitated with acetonitrile for sample preparation.
Result: The linear range was 1-3000 ng/mL with r>0.995, and LLOQ was 1 ng/mL. The intra-and inter-day precision of zhebeirine in mouse blood were less than 13%. The accuracy ranged from 91.2% to 112.5%, while the matrix effects were between 84.8% and 106.4%.
Conclusion: The UPLC-MS/MS was successfully applied to the pharmacokinetic study on zhebeirine after intravenous and oral administration, and the bioavailability was determined to be 22.8%.
Zhebeirine, Pharmacokinetics, Bioavailability, Mouse, UPLC-MS/MS, Blood
Department of Pharmacy, The First Affiliated Hospital of Hangzhou Normal University, Hangzhou 310000, Analytical and Testing Center, Wenzhou Medical University, Wenzhou 325035, Analytical and Testing Center, Wenzhou Medical University, Wenzhou 325035, Analytical and Testing Center, Wenzhou Medical University, Wenzhou 325035