Congcong Wen, Caiping Zhou, Yongxi Jin, Yujie Hu, Hongzhe Wang, Xianqin Wang* and Xuezhi Yang
Introduction: Epilepsy is one of the most common neurological diseases in clinical practice. The combined application of metabolomics technology plays a great advantage in the screening of biomarkers.
Method: In this study, Wistar rats were used as experimental subjects to model intractable epilepsy, to detect the metabolic changes of small molecules in plasma. UPLC-MS/MS was used to determinate the small molecules in rat plasma. UPLC HSS C18 (2.1mm×100mm, 1.7 μm) column was used for separation, column temperature of 40℃. The initial mobile phase was acetonitrile -0.3% formic acid with gradient elution, the flow rate was 0.3 mL/min, total running time 4.0 min. Quantitative analysis was performed with multi-response monitoring (MRM).
Result: Compare to the control group, the L-Alanine and L-Arginine decreased in the Epilepsy group (p<0.05); while Cytosine, Adenosine, L-Tyrosine, Citric acid, Fructose increased (p<0.05).
Conclusion: In the screening of epilepsy biomarkers using metabolomics, various amino acids that lead to increased energy production and neurotransmitter imbalance play an important role in epileptic seizures.
Epilepsy, plasma, UPLC-MS/MS, Amino acid, rat, metabolomics
Laboratory Animal Centre, Wenzhou Medical University, Wenzhou 325035, Laboratory Animal Centre, Wenzhou Medical University, Wenzhou 325035, Department of Rehabilitation, Wenzhou Municipal Hospital of Traditional Chinese Medicine, Wenzhou 325005, Laboratory Animal Centre, Wenzhou Medical University, Wenzhou 325035, Laboratory Animal Centre, Wenzhou Medical University, Wenzhou 325035, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000