Submit Manuscript  

Article Details


Determination of Meloxicam Using Direct and Indirect Flow Injection Spectrophotometry

Author(s):

Ream Ismail Abed and Hind Hadi*   Pages 1 - 11 ( 11 )

Abstract:


Background: Meloxicam is a non-steroidal anti-inflammatory drug, known to inhibit cyclooxygenase, a key enzyme of prostaglandin biosynthesis at the site of inflammation.

Objectives: Two sensitive and fast spectrophotometric methods (direct and indirect) using flow injection analysis (FIA) have been described for the assay of meloxicam in pure and in pharmaceutical formulations.

Methods: The direct method (method A) depended on the coupling of meloxicam with diazotized procaine benzylpenicillin in alkaline medium, while the indirect method (method B) involved a charge transfer reaction between alkaline hydrolytic product of meloxicam as n-donor with metol (p-methylaminophenol sulfate) as a π- acceptor using sodium periodate as an oxidant.

Results: The colored products exhibited absorption maxima at 492 and 656 nm for method A and B respectively. The methods obeyed Beer’s low over concentration ranges of 5-80 and 15-225 µg/mL, respectively. The detection limits were 2.73 and 5.26 µg/mL for direct and indirect methods, respectively. The chemical and physical parameters associated with FIA manifolds were optimized to construct the most sensitive and reproducible results.

Conclusion: Methods described herein are cheap, environmental friendly and showed no significant difference with those of official method indicated no significant difference in accuracy and precision.

Keywords:

Meloxicam, Procaine benzylpenicillin, Diazotization reaction, Charge transfer reaction, Metol, Flow injection

Affiliation:

Department of Chemistry, College of Science, University of Baghdad, Baghdad, Department of Chemistry, College of Science, University of Baghdad, Baghdad



Read Full-Text article