Ream Ismail Abed and Hind Hadi* Pages 1 - 11 ( 11 )
Background: Meloxicam is a non-steroidal anti-inflammatory drug, known to inhibit cyclooxygenase, a key enzyme of prostaglandin biosynthesis at the site of inflammation.
Objectives: Two sensitive and fast spectrophotometric methods (direct and indirect) using flow injection analysis (FIA) have been described for the assay of meloxicam in pure and in pharmaceutical formulations.
Methods: The direct method (method A) depended on the coupling of meloxicam with diazotized procaine benzylpenicillin in alkaline medium, while the indirect method (method B) involved a charge transfer reaction between alkaline hydrolytic product of meloxicam as n-donor with metol (p-methylaminophenol sulfate) as a π- acceptor using sodium periodate as an oxidant.
Results: The colored products exhibited absorption maxima at 492 and 656 nm for method A and B respectively. The methods obeyed Beer’s low over concentration ranges of 5-80 and 15-225 µg/mL, respectively. The detection limits were 2.73 and 5.26 µg/mL for direct and indirect methods, respectively. The chemical and physical parameters associated with FIA manifolds were optimized to construct the most sensitive and reproducible results.
Conclusion: Methods described herein are cheap, environmental friendly and showed no significant difference with those of official method indicated no significant difference in accuracy and precision.
Meloxicam, Procaine benzylpenicillin, Diazotization reaction, Charge transfer reaction, Metol, Flow injection
Department of Chemistry, College of Science, University of Baghdad, Baghdad, Department of Chemistry, College of Science, University of Baghdad, Baghdad