Submit Manuscript  

Article Details

Hydrophilic Interaction Liquid Chromatography (HILIC): Latest Applications in the Pharmaceutical Researches

[ Vol. 17 , Issue. 3 ]


Cem Erkmen, Weldejeworgıs Hndeya Gebrehiwot and Bengi Uslu*   Pages 316 - 345 ( 30 )


Background: Significant advances have been occurred in analytical research since the 1970s by Liquid Chromatography (LC) as the separation method. Reverse Phase Liquid Chromatography (RPLC) method, using hydrophobic stationary phases and polar mobile phases, is the most commonly used chromatographic method. However, it is difficult to analyze some polar compounds with this method. Another separation method is the Normal Phase Liquid Chromatography (NPLC), which involves polar stationary phases with organic eluents. NPLC presents low-efficiency separations and asymmetric chromatographic peak shapes when analyzing polar compounds. Hydrophilic Interaction Liquid Chromatography (HILIC) is an interesting and promising alternative method for the analysis of polar compounds. HILIC is defined as a separation method that combines stationary phases used in the NPLC method and mobile phases used in the RPLC method. HILIC can be successfully applied to all types of liquid chromatographic separations such as pharmaceutical compounds, small molecules, metabolites, drugs of abuse, carbohydrates, toxins, oligosaccharides, peptides, amino acids and proteins.

Objective: This paper provides a general overview of the recent application of HILIC in the pharmaceutical research in the different sample matrices such as pharmaceutical dosage form, plasma, serum, environmental samples, animal origin samples, plant origin samples, etc. Also, this review focuses on the most recent and selected papers in the drug research from 2009 to the submission date in 2020, dealing with the analysis of different components using HILIC.

Results and Conclusion: The literature survey showed that HILIC applications are increasing every year in pharmaceutical research. It was found that HILIC allows simultaneous analysis of many compounds using different detectors.


Analysis, applications, HILIC, liquid chromatography, pharmaceutical, separation.


Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, 06560, Ankara, Department of Forensic Chemistry and Toxicology, Institute of Forensic Sciences, Ankara University, 06590, Ankara, Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, 06560, Ankara

Graphical Abstract:

Read Full-Text article