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Bio-Analytical Method Development and Validation for Estimation of Zaltoprofen in Human Plasma by Reverse Phase -HPLC Method

Author(s):

Kishor Kumar Erukulla and Suseem S.R*   Pages 1 - 8 ( 8 )

Abstract:


Objective: A particular, easy and precise reverse phase liquid chromatographic technique was developed for the determination of Zaltoprofen in human plasma.

Methods: The ODS C18 (250mm x 4.6mm, 5µm) column was utilized for determination. The mobile phase contains buffer and acetonitrile (55:45 v/v). The rate of flow was 1.0 ml/min. The volume of sample infused was 10 µl. The column has been kept at a temperature of ~300C. The column was equilibrated for no less than 30 min prior to injection of the solutions. The detection wavelength was set at of 331 nm.

Results: The linearity experiment was performed for Zaltoprofen in the range of 0.15 to 20 µg/ml. The observed recovery of Zaltoprofen was 98.32 %.

Conclusion: The suggested technique has been validated and the same is very helpful for zaltoprofen determination in human plasma.

Keywords:

Zaltoprofen, Validation, Method, Buffer and Development.

Affiliation:

Department of Chemistry, VIT University, Vellore, Tamilnadu, Department of Chemistry, VIT University, Vellore, Tamilnadu



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