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Bio-Analytical Method Development and Validation for Estimation of Zaltoprofen in Human Plasma by Reverse Phase -HPLC Method


Kishor Kumar Erukulla and Suseem S.R*   Pages 1 - 8 ( 8 )


Objective: A particular, easy and precise reverse phase liquid chromatographic technique was developed for the determination of Zaltoprofen in human plasma.

Methods: The ODS C18 (250mm x 4.6mm, 5µm) column was utilized for determination. The mobile phase contains buffer and acetonitrile (55:45 v/v). The rate of flow was 1.0 ml/min. The volume of sample infused was 10 µl. The column has been kept at a temperature of ~300C. The column was equilibrated for no less than 30 min prior to injection of the solutions. The detection wavelength was set at of 331 nm.

Results: The linearity experiment was performed for Zaltoprofen in the range of 0.15 to 20 µg/ml. The observed recovery of Zaltoprofen was 98.32 %.

Conclusion: The suggested technique has been validated and the same is very helpful for zaltoprofen determination in human plasma.


Zaltoprofen, Validation, Method, Buffer and Development.


Department of Chemistry, VIT University, Vellore, Tamilnadu, Department of Chemistry, VIT University, Vellore, Tamilnadu

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