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Simultaneous Analysis of Sexual Stimulants and Anabolic Steroids as Adulterants in Dietary Supplements by High Performance Liquid Chromatography with Photodiode Array Detection

Author(s):

Carine Viana*, Gabriela Zanella Marcon, Thais Ramos Dal Molin, Denise Bohrer, Paulo Cícero do Nascimento and Leandro M. de Carvalho   Pages 1 - 7 ( 7 )

Abstract:


Background: The illegal virtual market for food supplements facilitates fraud and adulteration. Worldwide concern for consumer safety is growing on the part regulatory agencies, healthcare professionals and consumers.

Objective: This work aimed to evaluate the presence of sexual stimulants and anabolic steroids commonly used in the adulteration of dietary supplements through the development of a high performance liquid chromatography with photodiode array detection (HPLC-DAD) method for the identification and quantification of these compounds.

Method: The mobile phase composed of an ammonium acetate solution, acetonitrile and methanol leaded to the efficient separation of vardenafil, testosterone base, testosterone propionate, tadalafil, sildenafil and yohimbine.

Results: The assay was linear (r2> 0.999), precise (RSD% <0.5), accurate (99.1 to 105.2%), and the limits of detection and quantification were less than 0.05 and 0.15 μg/mL, respectively. Four samples of dietary supplements contained testosterone (n=1), tadalafil (n=2) and yohimbine (n=1) as adulterants. The adulterants found were in subtherapeutic doses, probably to reduce possible adverse effects and the action expected to appear natural. Since about 80% of adverse drug reactions are dose dependent. However, unpredictable adverse drug reactions are dose independent and based on idiosyncratic or allergic mechanisms or intolerance.

Conclusion: The developed method is convenient and easily applicable for adulteration detection of the analyzed drugs in the multicomponent supplements.

Keywords:

Adulterants, Anabolic Steroids, Dietary Supplements, Liquid Chromatography with Photodiode Array, Sexual Stimulants.

Affiliation:

Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria (UFSM), Santa Maria-RS, Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria (UFSM), Santa Maria-RS, Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria (UFSM), Santa Maria-RS, Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria (UFSM), Santa Maria-RS, Department of Chemistry, Federal University of Santa Maria (UFSM), Santa Maria-RS, Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria (UFSM), Santa Maria-RS



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