Swarupanjali Padhi*, Rupa Mazumder and Shradha Bisth Pages 1 - 5 ( 5 )
Background: In case of meningitis the meninges are inflamed and the Blood brain Barrier is distorted so there is no hindrance to drug penetration but the problem arises when the disease is at the verge of cure, the meninges become uninflamed and the permeability of drug is reduced to such extend the it becomes nearly impossible to maintain the minimum inhibitory concentration of drug at the site of infection. This problem was overcome by formulating Ceftriaxone loaded NLCs and administrating it through intraperitoneal route to Wistar Albino rat. For quantitative assessment of drug in rat serum and cerebrospinal spinal fluid new RPUFLC (reverse-phase ultra-fast liquid chromatography) method has been developed and validated.
Objective: Development and validation of RP UFLC (using ion pairing reagent) method for accurate estimation of ceftriaxone in rat serum and CSF.
Methods: Method validation is done according to ICH Guideline (Q2) for estimation of ceftriaxone in rat serum and its CSF (cerebrospinal fluid) by RP-UFLC method. The blood was collected from the rat tail vein and CSF collected carefully from cisterna magna of the rats by 23G syringe. Mobile phase used in a ratio of 70:30%v/v of phosphate buffer with ion-pairing reagent and acetonitrile with pH 8.0.
Results: Limit of detection and limit of quantification of ceftriaxone in rat serum was 1.08 µg/ml and 3.84 µg/ml respectively. Similarly, limit of detection and limit of quantification of ceftriaxone in CSF of rat was 0.94 µg/ml and 2.84 µg/ml respectively. In both cases the R2 value was more than 0.99 and showed 99% accuracy.
Conclusion: The experimental result suggests that the new RP-UFLC method developed and validated can be effectively used to assess ceftriaxone in preclinical studies in rats.
Ion-pairing reagent, Limit of detection, Limit of quantification, Recovery data.
Noida Institute of Engineering and Technology (Pharmacy Institute) Greater Noida, Gautam Buddha Nagar, Uttar Pradesh – 201306, Noida Institute of Engineering and Technology (Pharmacy Institute) Greater Noida, Gautam Buddha Nagar, Uttar Pradesh – 201306, Department of Pharmacy, Sardar Bhagwan Singh Post Graduate Institute of Biomedical Science & Research, Balawala, Dehradun, Uttarakhand – 248161