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Determination of three Unsaturated Fatty Acids in Pressure Ulcer Rats Using a UPLC-MS/MS Method

Author(s):

Fuman Cai, Yuwei Dong, Shaosheng Lou, Zeping Ma, Ting Wu, Liuqing Yang, Congcong Wen* and Chenxiang Wang   Pages 1 - 8 ( 8 )

Abstract:


Background: The serum levels of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and arachidonic acid (AA) under the state of pressure ulcers (PUs) are still unclear. Introduction: In order to investigate serum levels of DHA, EPA, and AA in PUs rats, an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS) method was developed and validated.

Methods: Chromatographic separation of DHA, EPA, AA was carried out on a BEH C18 column and gradient elute consisted of 5 mM ammonium acetate-0.1% formic acid and acetonitrile. Subsequently, fifty rats were divided into five groups (n=10), four PU groups (A-D) underwent various pressure and release time protocols, with group E as the control. The concentrations of DHA, EPA, AA from five groups were determination using validated method.

Results: The results showed there was good linearity for DHA (327.3/283.4), EPA (301.2/257.0), and AA (303.1/258.9) within 0.05-6.4 μg/mL. In control group, the levels of DHA, AA and EPA were 1.16±0.68, 0.59±0.19 and 0.78±0.21 μg/mL. At the end of modeling, concentrations of DHA, EPA and AA were increased after long and persistent pressure (>8 h). Especially, the level of DHA was significantly higher (P<0.01) than that of control group.

Conclusion: A stable, reliable and accurate UPLC-MS/MS for determination of DHA, EPA, AA in blood was developed. Serum concentrations of DHA, EPA and AA were altered differently after long and persistent pressure (>8 h), and DHA is a remarkable one.

Keywords:

UPLC-MS/MS, pressure ulcer, docosahexaenoic acid, eicosapentaenoic acid, arachidonic acid, rat

Affiliation:

School of Nursing, Laboratory Animal Center, Wenzhou Medical University, Wenzhou, School of Nursing, Laboratory Animal Center, Wenzhou Medical University, Wenzhou, School of Nursing, Laboratory Animal Center, Wenzhou Medical University, Wenzhou, School of Nursing, Laboratory Animal Center, Wenzhou Medical University, Wenzhou, School of Nursing, Laboratory Animal Center, Wenzhou Medical University, Wenzhou, Laboratory Animal Center, Wenzhou Medical University, Wenzhou, Laboratory Animal Center, Wenzhou Medical University, Wenzhou, Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou



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